General Information

Product

Viral Protein U Antibody

Description

Affinity Purified VPU Antibody

Verified Applications

CM, ELISA, Flow cytometry, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human Immunodeficiency Virus 1

Immunogen

Synthetic peptide taken within amino acid region 58-80 on VPU peptide. Synthetic peptide is covalently modified.

Sequence:

EGDQEELSALMEMGHHAPWNVND

Accession #

GenBank: BAD20654.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:50,000

ELISA

1:50,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q6L8A2

Overview

Cation Channel activity, Receptor Catabolic process, Viral release from host cell

Subcellular Location

Host cell membrane

Alternative Nomenclature

U ORF protein antibody

Vpu antibody

General Information

Product

CD86 Antibody Affinity Purified

Description

Affinity Purified T-lymphocyte activation antigen CD86 isoform 1 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-329 on human CD86 protein.

Accession #

NCBI:  NP_787058

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

P42081

Overview

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

Molecular Function

Receptor; Adaptive Immunity; Host-virus interaction; Immunity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Expressed by activated B-lymphocytes and monocytes.

Structure

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.  Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative Nomenclature

Activation B7 2 antigen antibody

B-lymphocyte activation antigen B7-2 antibody

B7 2 antibody

B70 antibody

BU63 antibody

CD 86 antibody

CD28 antigen ligand 2 2 antibody

CLS1 antibody

CTLA 4 counter receptor B7 2 antibody

Early T-cell costimulatory molecule 1 antibody

ETC-1 antibody

FUN 1 antibody

LAB72 antibody

Ly-58 antibody

MB7-2 antibody

Membrane glycoprotein antibody

MGC34413 antibody

T-lymphocyte activation antigen CD86 antibody

TS/A-2 antibody

General Information

Product

Sialoadhesin Antibody

Description

Affinity Purified CD169 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human Sialoadhesin protein.

Accession #

NCBI:  NP_075556

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9BZZ2

Overview

Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells.Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis.

Molecular Function

Carbohydrate binding; Cell adhesion; Endocytosis

Subcellular Location

Isoform 1: Cell membrane; Isoform 2: Secreted

Expression

Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophagrophages in rheumatoid arthritis.

Structure

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 16 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative Nomenclature

CD169 antibody

sialic acid binding Ig-like lectin 1, sialoadhesin antibody

Sialic acid-binding Ig-like lectin 1 antibody

Sialoadhesin antibody

Siglec 1 antibody

SN antibody

General Information

Product

Zika Virus NS5 Antibody

Description

Affinity Purified RNA-dependent RNA polymerase NS5 Antibody

Verified Applications

ELISA

Host

Rabbit

Species Cross Reactivity

Zika virus

Immunogen

Synthetic pepitde taken within amino acid region 3230-3280 on Zika virus NS5 protein.

Accession #

GenBank:  ALX35659.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Protein

Uniprot #

O55839

Overview

Viral RNA genome replication

Molecular Function

ATP binding; RNA-directed RNA polymerase activity

Alternative Nomenclature

NS5 zika virus antibody

RNA-dependent RNA polymerase NS5 antibody

nonstructural protein 5 antibody

General Information

Product

Zika Virus Envelope Protein E Antibody

Verified Applications

ELISA

Host

Rabbit

Species Cross Reactivity

Zika Virus

Immunogen

Synthetic peptide taken within amino acid region 280-320 on Zika Virus Envelope Protein E.

Accession #

NCBI:  YP_009227198

Uniprot:  UPI000764CF24

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

General Information

Product

Zika Virus P2K Antibody

Description

Affinity Purified Zika Virus Protein 2K Antibody

Verified Applications

ELISA

Host

Rabbit

Species Cross Reactivity

Zika Virus

Immunogen

23 mer Synthetic peptide taken from aa 2247 through 2269 of the ZIKA polyprotein AHL43505.

Accession #

NCBI:  AHL43505

Uniprot:  UPI000764CF24

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

General Information

Product

Zika Virus NS3 Antibody

Description

Affinity Purified Zika Virus Nonstructural protein NS3 Antibody

Verified Applications

ELISA

Host

Rabbit

Species Cross Reactivity

Zika Virus

Immunogen

Synthetic peptide taken within amino acid region 1-50 on Zika virus non structural protein NS3.

Accession #

NCBI:  YP_009227202

Uniprot:  UPI000764CEE5

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

General Information

Product

Zika Virus NS2 Antibody

Description

Affinity Purified Zika Virus Nonstructural protein NS2 Antibody

Verified Applications

ELISA

Host

Rabbit

Species Cross Reactivity

Zika Virus

Immunogen

Synthetic peptide taken within amino acid region 1300-1350 on Zika virus non structural protein NS2.

Accession #

GenBank: AHL43505

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

W8Q6T7

Overview

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Molecular Function

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Transferase

Subcellular Location

Virion membrane, Multi-pass membrane protein

Alternative Nomenclature

NS2 zika virus antibody
RNA-dependent RNA polymerase NS2 antibody
nonstructural protein 2 antibody

General Information

Product

Zika Virus NS1 Antibody

Description

Affinity Purified Zika Virus Nonstructural protein NS1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Zika Virus

Immunogen

Synthetic peptide taken within amino acid region 100-200 on Zika virus non structural protein NS1.

Accession #

NCBI:  YP_009227199.1

Uniprot:  UPI000764CEEF

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:250 - 1:500

General Information

Product

TSG101 Antibody

Description

Affinity Purified Tumor susceptibility gene 101 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-390 on Tumor susceptibility gene 101 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99816

Overview

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Molecular Function

calcium-dependent protein binding

DNA binding

ligand-dependent nuclear receptor transcription coactivator activity

protein homodimerization activity

transcription corepressor activity

ubiquitin binding

ubiquitin protein ligase binding

virion binding

Subcellular Location

Cytoplasm

Membrane; Peripheral membrane protein

Nucleus

Late endosome membrane; Peripheral membrane protein

Expression

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Alternative Nomenclature

ESCRT I complex subunit TSG101 antibody

TS101_HUMAN antibody

TSG 10 antibody

TSG 101 antibody

Tumor susceptibility gene 10 antibody

Tumor susceptibility gene 101 antibody

Tumor susceptibility protein antibody

Tumor susceptibility protein isoform 3 antibody

VPS23 antibody