General Information

Product

cIAP2 Antibody

Description

Affinity Purified baculoviral IAP repeat-containing protein 2 isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 545-620 on human cIAP2 protein.

Accession #

NCBI: NP_001157.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

Q134990

Molecular Function

Ligase activity; Transcription coactivator activity, Protein N-terminus binding

Subcellular Location

Cytoplasm; Nucleus

Expression

Is present in fetal and adult tissues and is mostly expressed in adult skeletal muscle, thymus, testis, ovary, and pancras.  Is low or absent in both brain and peripheral  blood leukocytes.

Structure

Interacts with DIABLO/SMAC. Interacts with CASP9.  Interacts with TRAF2; intercation required for IKBE ubiquination.

Alternative Nomenclature

AIP 1 antibody

API 2 antibody

Apoptosis inhibitor 2 antibody

Baculoviral IAP repeat containing 3 antibody

BIRC 3 antibody

HAIP 1 antibody

HIAP1 antibody

IAP homolog C antibody

MALT 2 antibody

Mammalian IAP homolog C antibody

MIHC antibody

RING finger protein 49 antibody

RNF49 antibody

TNFR2 TRAF signaling complex protein 1 antibody

General Information

Product

C9orf72 Antibody

Verified Applications

DB, ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on human C9orf72 isoform a protein.

Accession #

NCBI:  NP_001242983.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96LT7

Overview

May play a role in endosomal trafficking and autophagy

Molecular Function

Autophagy; endocytosis; cell death

Subcellular Location

Nucleus; Cytoplasm; Endosome; Lysosome; Autophagosome; Secreted

Expression

Both isoforms are widely expressed including kidney, lung, liver, heart, testis, cerebellum, frontal cortex and lymphoblasts.

Structure

Interacts with small Rab GTPases including RAB1A and RAB7A

Alternative Nomenclature

mALSFTD Antibody

chromosome 9 open reading frame 72 antibody

FTDals antibody

MGC23980 antibody

RP11-27J8.2 antibody

General Information

Product

FOXP3 Antibody

Description

Forkhead box protein 3 Antibody

Verified Applications

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Cat, Human, Monkey, Mouse, Rat

Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer

Immunogen

Synthetic peptide corresponding to amino acids 229-250 of Mouse Forkhead Box Protein 3 (winged helix transcription factor 3 protein). The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.

Sequence:

AQCLLQREVVQSLEQQLELEKE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyconal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:15,000-1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H5F2

Overview

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.

Molecular Function

Core promoter binding; Histone Acetyltransferase binding; Histone deacetylase binding; NFAT protein binding; NF-kappaB binding; Activator; Repressor

Subcellular Location

Nucleus; Cytoplasm

Expression

Down-regulated in regulatory T-cells (Treg) during inflammation.

Structure

Homodimer. Interacts with IKZF3. Isoform 1 (via LXXLL motif), but not isoform 2, interacts with isoform 4 of RORA (via AF-2 motif). Interacts with STUB1, HSPA8 and HSPA1A/B. Interacts with PPP1CA, PPP1CB and PPP1CG. Interacts with KAT5 and HDAC7. Interacts with HDAC9 in the absence of T-cell stimulation. Interacts with USP7. Interacts with isoform 2 of ZFP90 and can form a complex with TRIM28 in the presence of isoform 2 of ZFP90. Interacts with RUNX1. Interacts with RORC. Interacts with RELA and NFATC2. Interacts with RUNX2, RUNX3 and IKZF4.

Alternative Nomenclature

AIID antibody

DIETER antibody

Forkhead box P3 antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

IPEX antibody

JM2 antibody

MGC141961 antibody

MGC141963 antibody

PIDX antibody

SCURFIN antibody

XPID antibody

General Information

Product

HIF-1-Alpha isoform 1 Antibody

Description

Affinity Purified Hypoxia-inducible factor 1-alpha isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 790-826 on human HIF1A protein.

Accession #

NCBI:  NP_001521.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q16665

Overview

Functions as a master transcriptional regulator of the adaptive response to hypoxia.

Molecular Function

Activator

Subcellular Location

Cytoplasm, Nucleus

Expression

highest levels in kidney and heart;common human cancers and their metastases, due to the presence of intratumoral hypoxia

Structure

Heterodimer

Alternative Nomenclature

ARNT interacting protein antibody

Basic-helix-loop-helix-PAS protein MOP1 antibody

bHLHe78 antibody

Class E basic helix-loop-helix protein 78 antibody

HIF 1A antibody

HIF 1alpha antibody

Hypoxia inducible factor 1 alpha antibody

Member of PAS protein 1 antibody

Member of PAS superfamily 1 antibody

MOP1 antibody

PAS domain-containing protein 8 antibody

PASD8 antibody

General Information

Product

HIF-1-Alpha isoform 1 + 2 Antibody

Description

Affinity Purified Hypoxia-inducible factor 1-alpha isoform 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken wtihin amino acid region 150-250 on human HIF1A protein.

Accession #

NCBI:  NP_851397.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q16665

Overview

Functions as a master transcriptional regulator of the adaptive response to hypoxia.

Molecular Function

Activator

Subcellular Location

Cytoplasm, Nucleus

Expression

highest levels in kidney and heart;common human cancers and their metastases, due to the presence of intratumoral hypoxia

Structure

Heterodimer

Alternative Nomenclature

ARNT interacting protein antibody

Basic-helix-loop-helix-PAS protein MOP1 antibody

bHLHe78 antibody

Class E basic helix-loop-helix protein 78 antibody

HIF 1A antibody

HIF 1alpha antibody

Hypoxia inducible factor 1 alpha antibody

Member of PAS protein 1 antibody

Member of PAS superfamily 1 antibody

MOP1 antibody

PAS domain-containing protein 8 antibody

PASD8 antibody

General Information

Product

IRE1A Antibody

Description

Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody

Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
inositol-requiring enzyme 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody

MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody

General Information

Product

DRIP130 Antibody

Description

Affinity Purified MED23 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic pepetide taken within amino acid region 650-700 on MED23 protein.

Accession #

GenBank: AAH60759.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9ULK4

Overview

Required for transcriptional activation subsequent to assembly of preinitiation complex. Component of mediator complex, mediator functions as a bridge to convey information from gene specific regulatory proteins to basal RNA polymerase II transcription machinery.

Molecular Function

Transcription coactivator activity

Subcellular Location

Nucleus

Alternative Nomenclature

130 kDa transcriptional co activator antibody

ARC 130 antibody

Cofactor required for Sp1 transcriptional activation subunit 3 130kDa antibody

CRSP 130 antibody

CRSP 133 antibody

DRIP 130 antibody

KIAA1216 antibody

MED 23 antibody

SUR 2 antibody

Vitamin D3 receptor interacting protein antibody

General Information

Product

MEDEA Antibody Affinity Purified

Description

Affinity Purified Medea Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 150-250 on Drosophila melanogaster Medea protein.

Accession #

GenBank: AAC62005.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.73 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q7KPL1

Overview

Sequence-specific DNA binding transcription factor activity

Molecular Function

Transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Subcellular Location

Cytoplasm;Nucleus

Structure

Belongs to the dwarfin/SMAD family.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody

Deleted in Pancreatic Carcinoma 4 antibody

DPC 4 antibody

hSMAD4 antibody

JIP antibody

MAD mothers against decapentaplegic homolog 4 antibody

MADH 4 antibody

Med antibody

Medea antibody

MYHRS antibody

Smad 4 antibody

General Information

Product

NM23A Antibody Affinity Purified

Description

Affinity Purified Nucleoside diphosphate kinase A Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human NDKA protein.

Accession #

GenBank: AAO85436.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P15531

Overview

Major role in the synthesis of nucleoside triphosphates other than ATP.

Molecular Function

Kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen,thymus and lung carcinoma cell lines

Structure

Hexamer of two different chains: A and B

Alternative Nomenclature

AWD antibody

GAAD antibody

Granzyme A activated DNase antibody

Metastasis inhibition factor nm23 antibody

NB antibody

NDK A antibody

NM23 antibody

NME 1 antibody

Non metastatic cells 1 protein antibody

Nonmetastatic protein 23 antibody

Nucleoside diphosphate kinase A antibody

Tumor metastatic process associated protein antibody

General Information

Product

NME2 + NM23A Antibody

Description

Affinity Purified Nucleoside diphosphate kinase A and Nucleoside diphosphate kinase B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 100-150 on NM23A protein selective for both NDKA and NDKB.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P15531

Overview

Major role in the synthesis of nucleoside triphosphates other than ATP.

Molecular Function

Kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen,thymus and lung carcinoma cell lines

Structure

Hexamer of two different chains: A and B

Alternative Nomenclature

C myc purine binding transcription factor PUF antibody

Histidine protein kinase NDKB antibody

MGC111212 antibody

MGC2212 antibody

NDK B antibody

NM23B antibody

nme2 antibody

Nucleoside diphosphate kinase B antibody

PUF antibody