General Information

Product

THAP1 Antibody

Description

Affinity Purified THAP domain-containing protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-210 on human THAP domain-containing protein 1.

Accession #

GenBank: CAG33537

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NVV9

Overview

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.

Molecular Function

Identical protein binding, Sequence-specific DNA binding, Zinc ion binding

Subcellular Location

Nucleus > nucleoplasm, PML body

Expression

Highly expressed in heart, skeletal muscle, kidney and liver. Weaker expression in brain and placenta.

Structure

Belongs to the THAP1 family.

Alternative Nomenclature

Nuclear proapoptotic factor antibody

THAP 1 antibody

THAP domain containing apoptosis associated protein 1 antibody

THAP domain containing protein 1 antibody

THAP domain protein 1 antibody

General Information

Product

DNA PKcs Antibody

Description

Affinity Purified DNA-dependent protein kinase catalytic subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 4000-4128 of human DNPK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P78527

Overview

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus

Expression

Found in axons of the central and peripheral nervous system;differentiated cell types of the intestine

Structure

Belongs to the immunoglobulin superfamily. DCC family;Contains 6 fibronectin type-III domains;Contains 4 Ig-like C2-type (immunoglobulin-like) domains

Alternative Nomenclature

DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-PKcs antibody
DNAPK antibody
DNAPK catalytic subunit antibody
DNPK1 antibody
Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
HYRC antibody
HYRC1 antibody
p350 antibody
p460 antibody
PKRDC antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC7 antibody

General Information

Product

PERK Antibody

Description

Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.

Accession #

NCBI:  NP_004827

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9NZJ5

Overview

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Expression

Ubiquitous. A high level expression is seen in secretory tissues.

Structure

Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.

Alternative Nomenclature

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

PEK antibody

PRKR like endoplasmic reticulum kinase antibody

WRS antibody

General Information

Product

Phospho-RBM4 Antibody

Description

Affinity Purified RNA binding motif protein 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 300-350 on human RNA binding motif protein 4.

Accession #

GenBank: AAH32735

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9BWF3

Overview

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin.

Molecular Function

cyclin binding

miRNA binding

mRNA 3'-UTR binding

mRNA binding

pre-mRNA intronic binding

pre-mRNA intronic pyrimidine-rich binding

RNA binding

zinc ion binding

Subcellular Location

Nucleus, Cytoplasm

Structure

Repeat, Zinc-finger

Alternative Nomenclature

dkfzp547k0918 antibody

hLark antibody

lark antibody

Lark homolog antibody

mgc75138 antibody

RBM4 antibody

rbm4 lark antibody

RNA binding motif 4 antibody

RNA-binding motif protein 4a antibody

RNA-binding protein 4 antibody

General Information

Product

GPS2 Antibody

Description

Affinity Purified G protein pathway suppressor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human G protein pathway suppressor 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q13227

Molecular Function

cyclin binding

GTPase inhibitor activity

transcription coactivator activity

transcription corepressor activity

Subcellular Location

Nucleus, Cytosol

Expression

Widely expressed.

Structure

Coiled coil

Alternative Nomenclature

AMF 1 antibody

G protein pathway suppressor 2 antibody

GPS 2 antibody

General Information

Product

SRPK1 Antibody

Description

Affinity Purified SRSF protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 405-455 on human SRSF protein kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q96SB4

Molecular Function

ATP binding

magnesium ion binding

protein kinase activity

protein serine/threonine kinase activity

RNA binding

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus

Structure

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.

Alternative Nomenclature

Serine/arginine rich protein specific kinase 1 antibody
Serine/threonine protein kinase SRPK1 antibody
SFRS protein kinase 1 antibody
SFRSK1 antibody
SR protein kinase 1 antibody
SR protein specific kinase 1 antibody
SRPK1 antibody

General Information

Product

PTBP1 Antibody

Description

Affinity Purified Polypyrimidine tract-binding protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 150-200 on human Polypyrimidine tract-binding protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P26599

Overview

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).

Molecular Function

mRNA binding

poly-pyrimidine tract binding

pre-mRNA binding

RNA binding

Subcellular Location

Nucleus

Structure

Monomer.

Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1.

Interacts with RAVER1 and SFPQ.

Alternative Nomenclature

57 kDa RNA-binding protein PPTB-1 antibody

Heterogeneous nuclear ribonucleoprotein I antibody

Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody

HNRPI antibody

MGC10830 antibody

MGC8461 antibody

Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody

Polypyrimidine Tract Binding Protein 1 antibody

pPTB antibody

PTB 1 antibody

PTB 2 antibody

PTB 3 antibody

PTB 4 antibody

PTB antibody

PTB T antibody

PTBP 1 antibody

PTBP1 antibody

PTBP1_HUMAN antibody

PTBT antibody

RNA Binding Protein antibody