General Information

Product

AGE Antibody

Description

Affinity Purified Advance Glycated Endproduct Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda

Immunogen

Purified glycated ribonuclease corresponding to AGE. Glycated ribonuclease (RNase) prepared by incubating RNase with glucose at 37⁰C for several months.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Overview

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators.

Molecular Function

Inflammatory Response

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Secreted

Isoform 10 : Cell membrane 1 Publication; Single-pass type I membrane protein

Expression

Endothelial cells.

Structure

Interacts with S100A1 and APP (By similarity). Interacts with S100B, S100A12 and S100A14. Constitutive homodimer; disulfide-linked.

Alternative Nomenclature

Advanced glycation end products antibody

AGEs antibody

General Information

Product

CCR2 Antibody

Description

Affinity Purified C-C chemokine receptor type 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 20-70 on human CCR2 protein.

Accession #

NCBI: NP_955783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.71 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P41597

Overview

Receptor for CCL2, CCL7 and CCL13 chemokines.  Transduces a signal by increasing intracellular calcium ion levels.  Alternative coreceptor with CD4 for HIV-1 infection.

Molecular Function

Host-virus interaction; GPCR; Transducer

Subcellular Location

Cell membrane; Mulipass membrane protein.

Expression

Induced; Up-regulated by CREB3

Structure

Binds to HIV-1 Tat.  Interacts with ARRB1

Alternative Nomenclature

CC CKR 2 antibody

CD192 antibody

CKR2A antibody

CKR2B antibody

CMKBR2 antibody

MCP1 RECEPTOR antibody

Monocyte chemoattractant protein 1 receptor antibody

General Information

Product

CCR4 Antibody

Description

Affinity Purified C-C chemokine receptor type 4 Antibody

Verified Applications

CF, DB, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human C-C chemokine receptor type 4 protein.

Accession #

NCBI: NP_005499

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P51679

Overview

Receptor for C-C type chemokines CCL17/TARC and CCL22/MDC.  Functions as chemoattractant homing receptor on circulating memory lymphocytes and as coreceptor for some primary HIV-2 isolates.  Can also mediate hippocampal-neuron survival.

Molecular Function

GPCR, Receptor, Transducer.

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Thymus, peripheral blood leukocytes including T-cells, CD4+ cells, basophils and in platelets.  Expressed less in spleen and in monocytes.  Also found in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expresseding cutaneous lymphocyte antigen.  Expressed in brain and microvascular and coronary artery endothelial cells.

Structure

Belongs to G-protein coupled receptor 1 family.  Interacts with CREB3.

Alternative Nomenclature

CD194 antibody

ChemR13 antibody

CKR4 antibody

CMKBR 4 antibody

HGCN 14099 antibody

K5 5 antibody

General Information

Product

CD36 Antibody Affinity Purified

Description

Affinity Purified platelet glycoprotein 4 isoform 1 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human CD36 protein.

Accession #

NCBI:  NP_001120916.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P16671

Overview

Involved in binding to collagen, thrombospodin, anionic phospholipids and oxidized low density lipoprotein.  Serves to bind long chain fatty acids and  also may act as a cell adhesion molecule, or regulator of fatty acid transport.

Molecular Function

Lipoprotein particle binding; thrombospondin receptor activity; Receptor

Subcellular Location

Membrane; Multipass membrane protein

Structure

Interacts with THBS1 and THBS2, mediating THBS antiangiogenic activity.

Alternative Nomenclature

Adipocyte membrane protein antibody

thrombospondin receptor) antibody

cd36 antigen antibody

CHDS7 antibody

Cluster determinant 36 antibody

Collagen receptor, platelet antibody

FAT antibody

fatty acid translocase antibody

glycoprotein iiib antibody

GP IIIb antibody

gp4 antibody

gpiv antibody

leukocyte differentiation antigen cd36 antibody

PAS 4 protein antibody

platelet collagen receptor antibody

scarb3 antibody

Scavenger receptor class B member 3 antibody

thrombospondin receptor antibody

General Information

Product

Glucose Transporter GLUT1 Antibody

Verified Applications

ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB, Immunomicroscopy

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1. Peptide was covalently modified.

Sequence:

PEELFHPLGADSQV

Accession #

GenBank: AAC28635.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:12,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:5,000

Protein

Uniprot #

P11166

Overview

Facilitative glucose transporter.  May be responsible for constitutive or basal glucose uptake.

Molecular Function

Sugar Transport

Subcellular Location

Cell membrane; Multi-pass membrane protein; melanosome

Expression

Detected in erythrocytes.  Variable expression in many human tissues

Structure

Transmembrane helix

Alternative Nomenclature

DYT17 antibody

DYT18 antibody

erythrocyte/brain antibody

Erythrocyte/brain HepG2 glucose transporter antibody

Erythrocyte/hepatoma glucose transporter antibody

facilitated glucose transporter member 1 antibody

Glucose transporter 1 antibody

GLUT1 antibody

GLUT1DS antibody

GLUTB antibody

GT1 antibody

GTG1 antibody

Gtg3 antibody

GTR1_HUMAN antibody

HepG2 glucose transporter antibody

PED antibody

RATGTG1 antibody

SLC2A1 antibody

Solute carrier family 2 (facilitated glucose transporter), member 1 antibody

General Information

Product

GLUT12 Antibody

Description

Affinity Purified Solute carrier family 2 facilitated glucose transporter member 12 Antibody

Verified Applications

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide from the C-terminus of Rat GLUT12. 

Accession #

GenBank: EDL87731.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:4500

ELISA

1:4500

Immunoprecipitation

1:200

Western Blot

1:500. Predicted molecular weight 67 kDa.

Protein

Uniprot #

D3ZNG4

Overview

Substrate-specific transmembrane transporter activity

Molecular Function

Sugar Transport

Subcellular Location

Integral component of membrane

Structure

Belongs to major facilitator superfamily Sugart Transporter family.

Alternative Nomenclature

Glucose transporter type 12 antibody

GLUT8 antibody

SLC2A12 antibody

Solute carrier family 2 (facilitated glucose transporter) member 12 antibody

General Information

Product

Junctional Adhesion Molecule 1 Antibody

Description

Affinity Purified Junctional adhesion molecule A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Monkey, Mouse, Panda, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-299 on human Junctional Adhesion Molecule A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y624

Overview

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3.  Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.

Subcellular Location

Cell junction, cell membrane, single-pass type I membrane protein (Note: Localized at tight junctions of both epithelial and endothelial cells)

Alternative Nomenclature

9130004G24 antibody

AA638916 antibody

CD 321 antibody

ESTM33 antibody

F11 receptor antibody

F11R antibody

JAM 1 antibody

JAM A antibody

JCAM antibody

Junction adhesion molecule 1 antibody

KAT antibody

Ly106 antibody

PAM1 antibody

Platelet adhesion molecule 1 antibody

Platelet F11 receptor antibody

PRO301 antibody

UNQ264/PRO301 antibody

General Information

Product

MyD88 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 140-190 on human MyD88 protein.

Accession #

GenBank: AAC50954.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q99836

Overview

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.

Molecular Function

death receptor binding;TIR domain binding

Subcellular Location

Cytoplasm

Expression

Ubiquitous

Structure

Homodimer. Also forms heterodimers with TIRAP

Alternative Nomenclature

MYD 88 antibody

Myeloid differentiation marker 88 antibody

General Information

Product

Norepinephrine Transporter Antibody

Description

Affinity Purified Sodium -dependent noradrenaline transporter Antibody

Verified Applications

Dot Blot, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N-terminal amino acids that are unique to noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage Instructions

Shipped at 4⁰C. Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid repeated freeze / thaw cycles.

pH: 7.80
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris glycine, 30% Glycerol

Application Protocol

Confocal Microscopy

1:100

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

P23975

Overview

Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.

Molecular Function

monoamine transmembrane transporter activity; norepinephrine:sodium symporter activity

Subcellular Location

Membrane

Expression

Adrenal gland; Placenta; Testis; Skin

Structure

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Alternative Nomenclature

NAT1 antibody

NET antibody

NET1 antibody

Neurotransmitter transporter antibody

Norepinephrine transporter antibody

SLC6A2 antibody

SLC6A5 antibody

Sodium dependent noradrenaline transporter antibody

Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibody

Solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibody

Solute carrier family 6 member 2 antibody

Solute carrier family 6 member 5 antibody

General Information

Product

NPY2R Antibody

Description

Affinity Purified Neuropeptide Y receptor Y2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-381 on human NPY2R protein.

Accession #

GenBank: AAO92062.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P49146

Overview

Receptor for neuropeptide Y and peptide YY

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Membrane

Expression

High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.

Structure

Belongs to the G-protein coupled receptor 1 family

Alternative Nomenclature

Neuropeptide Y Receptor Type 2 antibody

NPY2R antibody

NPYY2 Receptor antibody

Y2 receptor antibody