General Information

Product

ATG16L1 Antibody

Description

Affinity Purified Autophagy-related protein 16-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q676U5

Overview

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Molecular Function

Identical protein binding, Ubiquitin-like protein transferase activity

Subcellular Location

Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein

Structure

Homooligomer. Interacts with WIPI2, FIP200 and ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts with TMEM

Alternative Nomenclature

APG16 like 1 antibody

APG16L antibody

APG16L beta antibody

ATG16 autophagy related 16 like 1 antibody

ATG16A antibody

ATG16L antibody

Autophagy related protein 16 1 antibody

IBD10 antibody

WD repeat domain 30 antibody

WDR30 antibody

General Information

Product

TSG101 Antibody

Description

Affinity Purified Tumor susceptibility gene 101 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-390 on Tumor susceptibility gene 101 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99816

Overview

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Molecular Function

calcium-dependent protein binding

DNA binding

ligand-dependent nuclear receptor transcription coactivator activity

protein homodimerization activity

transcription corepressor activity

ubiquitin binding

ubiquitin protein ligase binding

virion binding

Subcellular Location

Cytoplasm

Membrane; Peripheral membrane protein

Nucleus

Late endosome membrane; Peripheral membrane protein

Expression

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Alternative Nomenclature

ESCRT I complex subunit TSG101 antibody

TS101_HUMAN antibody

TSG 10 antibody

TSG 101 antibody

Tumor susceptibility gene 10 antibody

Tumor susceptibility gene 101 antibody

Tumor susceptibility protein antibody

Tumor susceptibility protein isoform 3 antibody

VPS23 antibody

General Information

Product

KK-LC-1 Antibody

Description

Affinity Purified Kita-kyushu lung cancer antigen 1 Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 20-70 and 61-111 on human Kita-kyushu lung cancer antigen 1.

Accession #

NCBI:  NP_001017978

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multiple epitopes

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q5H943

Subcellular Location

Cell membrane; Single-pass type II membrane protein

Expression

Specifically expressed in testis. Expressed by cancer cell lines.

Alternative Nomenclature

Cancer/testis antigen 83 antibody

Chromosome X open reading frame 61 antibody

CT83 antibody

CXorf61 antibody

Kita-kyushu lung cancer antigen 1 antibody

KK-LC-1 antibody

KKLC1 antibody

General Information

Product

Phospho-STAT6 Antibody

Description

Affinity Purified Phospho-specific Signal transducer and activator of transcription 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 610-660 on human Phospho-specific Signal transducer and activator of transcription 6  protein. Phosphorylated Tyrosine 641 residue.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P42226

Overview

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Subcellular Location

Nucleus

Structure

Belongs to the transcription factor STAT family.

Alternative Nomenclature

IL4 STAT antibody

Interleukin 4 Induced antibody

Interleukin 4 Induced Transcription Factor IL4 STAT antibody

Signal transducer and activator of transcription 6 antibody

Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody

Signal transducer and activator of transcription 6, interleukin 4 induced antibody

STAT 6 antibody

STAT interleukin4 induced antibody

Stat6 antibody

STAT6B antibody

STAT6C antibody

Transcription factor IL 4 STAT antibody

General Information

Product

Elf4/MEF Antibody

Description

Affinity Purified ETS-related transcription factor Elf-4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 605-655 on mouse ETS-related transcription factor Elf-4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9Z2U4

Molecular Function

RNA polymerase II proximal promoter sequence-specific DNA binding

Subcellular Location

Nucleus > PML body

Alternative Nomenclature

E74-like factor 4 antibody

ELF4 antibody

ELFR antibody

ETS-related transcription factor Elf-4 antibody

MEF antibody

Myeloid Elf-1-like factor antibody