General Information

Product

MERTK Antibody

Description

Affinity Purified Human cellular proto-oncogene (c-mer) mRNA Antibody N-epitope

Verified Applications

CM, ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on MerTK protein.

Accession #

NCBI:  NP_075232

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:750

Protein

Uniprot #

P57097

Overview

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3 (By similarity).

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Is expressed in many neoplastic B- and T-cells.  Is expressed highly in testis, ovary, prostate, lung and kidney and is expressed at low levels in the spleen, small intestine, colon and liver.

Structure

Interacts with TNK2 upon activation.  Interacts with extracellular ligands LGALS3, TUB, TULP1, and GAS6.

Alternative Nomenclature

c MER antibody

c mer proto oncogene tyrosine kinase antibody

Eyk antibody

MER antibody

MER receptor tyrosine kinase antibody

MERK antibody

MERPEN antibody

Mertk antibody

MERTK c-mer proto-oncogene tyrosine kinase antibody

MGC133349 antibody

nmf12 antibody

Nyk antibody

Proto oncogene tyrosine protein kinase MER antibody

Proto-oncogene c-Mer antibody

Receptor tyrosine kinase MerTK antibody

RP38 antibody

STK kinase antibody

Tyrosine-protein kinase Mer antibody

General Information

Product

MERTK Antibody

Description

Affinity Purified Human cellular proto-oncogene (c-mer) Antibody Mid-Region

Verified Applications

CM, ELISA, Flow Cytometry, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide:

VQETKFGGAFSEEDC

corresponding to amino acid region 524-537 on Rat MERTK protein.

Accession #

NCBI:  NP_075232

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:750

Protein

Uniprot #

P57097

Overview

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Is expressed in many neoplastic B- and T-cells.  Is expressed highly in testis, ovary, prostate, lung and kidney and is expressed at low levels in the spleen, small intestine, colon and liver.

Structure

Interacts with TNK2 upon activation.  Interacts with extracellular ligands LGALS3, TUB, TULP1, and GAS6.

Alternative Nomenclature

c MER antibody

c mer proto oncogene tyrosine kinase antibody

Eyk antibody

MER antibody

MER receptor tyrosine kinase antibody

MERK antibody

MERPEN antibody

Mertk antibody

MERTK c-mer proto-oncogene tyrosine kinase antibody

MGC133349 antibody

nmf12 antibody

Nyk antibody

Proto oncogene tyrosine protein kinase MER antibody

Proto-oncogene c-Mer antibody

Receptor tyrosine kinase MerTK antibody

RP38 antibody

STK kinase antibody

Tyrosine-protein kinase Mer antibody

General Information

Product

MERTK Antibody

Description

Affinity Purified Human cellular proto-oncogene (c-mer) mRNA Antibody C-epitope

Verified Applications

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-994 on MerTK protein.

Accession #

NCBI:  NP_075232

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:750-1:2,000

Protein

Uniprot #

P57097

Overview

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Is expressed in many neoplastic B- and T-cells.  Is expressed highly in testis, ovary, prostate, lung and kidney and is expressed at low levels in the spleen, small intestine, colon and liver.

Structure

Interacts with TNK2 upon activation.  Interacts with extracellular ligands LGALS3, TUB, TULP1, and GAS6.

Alternative Nomenclature

c MER antibody

c mer proto oncogene tyrosine kinase antibody

Eyk antibody

MER antibody

MER receptor tyrosine kinase antibody

MERK antibody

MERPEN antibody

Mertk antibody

MERTK c-mer proto-oncogene tyrosine kinase antibody

MGC133349 antibody

nmf12 antibody

Nyk antibody

Proto oncogene tyrosine protein kinase MER antibody

Proto-oncogene c-Mer antibody

Receptor tyrosine kinase MerTK antibody

RP38 antibody

STK kinase antibody

Tyrosine-protein kinase Mer antibody

General Information

Product

GCN2 Antibody

Description

Affinity Purified eIF2alpha kinase Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1700 on human GCN2 protein.

Accession #

GenBank:  CAB58363.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9P2K8

Overview

Can phosphorylate alpha subunit of EIF2 and mediates translational control.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Cytosolic ribosome

Expression

Liver, brain.

Structure

Contains 2 protein kinase domains; Contains 1 RWD domain.

Alternative Nomenclature

Eif2ak4 antibody

Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody

GCN2 antibody

GCN2 eIF2alpha kinase antibody

GCN2 like protein antibody

KIAA1338 antibody

MGCN2 antibody

General Information

Product

GCN2 Antibody

Description

Affinity Purified eIF2alpha kinase Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1700 on human GCN2 protein.

Accession #

GenBank:  CAB58363.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9P2K8

Overview

Can phosphorylate alpha subunit of EIF2 and mediates translational control.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Cytosolic ribosome

Expression

Liver, brain.

Structure

Contains 2 protein kinase domains; Contains 1 RWD domain.

Alternative Nomenclature

Eif2ak4 antibody

Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody

GCN2 antibody

GCN2 eIF2alpha kinase antibody

GCN2 like protein antibody

KIAA1338 antibody

MGCN2 antibody

General Information

Product

MAP3K10 Antibody 

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 10 Antibody

Verified Applications

DB, ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide correpsonding to the C-terminal taken within amino acid region 900-950 on human MAP3K10 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02779

Overview

Activates the JUN N-terminal pathway.

Molecular Function

ATP binding; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm

Expression

Expressed in brain and skeletal muscle.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Alternative Nomenclature

MEKK10 antibody

MEKK9 antibody

Mitogen activated protein kinase kinase kinase 10 antibody

Mitogen activated protein kinase kinase kinase 9 antibody

Mixed lineage kinase 2 antibody

MKN28 derived nonreceptor type serine/threonine kinase antibody

MKN28 kinase antibody

MLK1 antibody

MLK2 antibody

MST antibody

PRKE1 antibody

Protein kinase MST antibody

General Information

Product

FAK1 Antibody

Description

Affinity Purified Focal Adhesion Kinase 1

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 740-790 on human focal adhesion kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q05397

Overview

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Molecular Function

Developmental protein; Kinase; Transferase; Tyrosine-protein kinase

Subcellular Location

Cell junction › focal adhesion

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasm › cell cortex

Cytoplasm › cytoskeleton

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Nucleus

Expression

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

FADK 1 antibody

FADK antibody

FAK 1 antibody

FAK related non kinase polypeptide antibody

FAK1 antibody

Focal adhesion kinase 1 antibody

Focal adhesion kinase isoform FAK Del33 antibody

Focal adhesion kinase related nonkinase antibody

FRNK antibody

p125FAK antibody

pp125FAK antibody

PPP1R71 antibody

Protein phosphatase 1 regulatory subunit 71 antibody

Protein tyrosine kinase 2 antibody

Protein Tyrosine Kinase Cytoplasmic antibody

PTK 2 antibody

PTK2 protein tyrosine kinase 2 antibody

General Information

Product

SGK2 Antibody

Description

Affinity Purified Serine/Threonine-protein Kinase Sgk2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 377-427 on human Sgk2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9HBY8

Overview

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na+ channels: SCNN1A/ENAC, K+ channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase.

Molecular Function

ATP-binding, Nucleotide binding; Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Nucleus

Expression

Highly expressed in liver, kidney and pancreas, and at lower levels in brain.

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

DJ138B7.2 antibody
HSGK2 antibody
Serine/threonine protein kinase SGK2 antibody

Serum/glucocorticoid regulated kinase 2 antibody

SGK2 antibody

General Information

Product

SGK3 Antibody

Description

Affinity Purified Serine/Threonine-protein Kinase Sgk3 Antibody

Verified Applications

CM, ELISA, IC, IF, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 440-490 on human Sgk3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q96BR1

Overview

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na+ channels: SCNN1A/ENAC and SCN5A, K+ channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.

Molecular Function

ATP-binding, Nucleotide binding; Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasmic vesicle; Early endosome; Recycling endosome

Expression

Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 PX (phox homology) domain.

Alternative Nomenclature

CISK antibody

Cytokine independent survival kinase antibody

DKFZp781N0293 antibody

Serine/threonine protein kinase Sgk3 antibody

Serum/glucocorticoid regulated kinase family member 3 antibody

Serum/glucocorticoid-regulated kinase 3 antibody

SGK 3 antibody

Sgk3 antibody

SGKL antibody

General Information

Product

DCK Antibody

Description

Affinity Purified Deoxycytidine kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 210-260 on mouse Deoxycytidine kinase.

Accession #

NCBI:  NP_031858.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P43346

Overview

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).

Molecular Function

ATP binding; deoxycytidine kinase activity; drug binding; nucleoside kinase activity; protein homodimerization activity

Subcellular Location

Nucleus

Structure

Homodimer; Belongs to the DCK/DGK family.

Alternative Nomenclature

DCK protein antibody

Deoxycytidine kinase antibody

Deoxynucleoside kinase antibody

EC 2.7.1 antibody

EC 2.7.1.74 antibody

MGC117410 antibody

MGC138632 antibody