General Information

Product

BACE2 B Antibody

Description

Affinity Purified beta-secretase 2 isoform B preproprotein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 350-396 on human BACE2B protein.

Accession #

NCBI:  NP_620477

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y5Z0

Overview

Responsible for the proteolytic processing of the amyloid precursor protein. Cleaves APP between 690 and 691 leading to the generation and extracellular release of beta-cleaved soluble APP and C-terminal fragment which is later released by gamma-secretase.

Molecular Function

Aspartic-type endopeptidase activity.

Subcellular Location

Endoplasmic reticulum, Endosome, Golgi apparatus; Membrane

Expression

Neurons within the hippocampus, frontal cortex and temporal cortex. Lower expression in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Also found in spinal cord, medulla oblongata, substania nigra and locus coruleus. Expressed in ductal epithelium of prostate.

Structure

Belongs to the peptidase A1 family.

Alternative Nomenclature

ALP 56 antibody

ASP1 antibody

ASP21 antibody

Aspartic Like Protease 56kDa antibody

Downs Syndrome Region Aspartic Protease antibody

DRAP antibody

Memapsin-1 antibody

Membrane associated aspartic protease 1 antibody

Theta-secretase antibody

General Information

Product

BACE2 A/C Antibody

Description

Affinity Purified Beta Secretase beta-site APP cleaving Enzyme 2A/C Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 490-518 on human BACE2 protein.

Accession #

GenBank: AAQ89286

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.76 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y5Z0

Overview

Responsible for the proteolytic processing of the amyloid precursor protein. Cleaves APP between 690 and 691 leading to the generation and extracellular release of beta-cleaved soluble APP and C-terminal fragment which is later released by gamma-secretase.

Molecular Function

Aspartic-type endopeptidase activity.

Subcellular Location

Endoplasmic reticulum, Endosome, Golgi apparatus; Membrane

Expression

Neurons within the hippocampus, frontal cortex and temporal cortex. Lower expression in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Also found in spinal cord, medulla oblongata, substania nigra and locus coruleus. Expressed in ductal epithelium of prostate.

Structure

Belongs to the peptidase A1 family.

Alternative Nomenclature

ALP 56 antibody

ASP1 antibody

ASP21 antibody

Aspartic Like Protease 56kDa antibody

Downs Syndrome Region Aspartic Protease antibody

DRAP antibody

Memapsin-1 antibody

Membrane associated aspartic protease 1 antibody

Theta-secretase antibody

General Information

Product

FAM38A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken between TMD14 and TMD15 on human FAM38A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q92508

Overview

Pore-forming subunit of a mechanosensitive non-specific cation channel;positive regulation of cell-cell adhesion mediated by integrin

Molecular Function

Ion channel

Subcellular Location

Cell membrane, Endoplasmic reticulum, Membrane

Expression

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes;Expressed in erythrocytes

Structure

Homooligomer, most likely homotetramer

Alternative Nomenclature

DHS antibody

Fam38a antibody

Family with sequence similarity 38 member A antibody

KIAA0233 antibody

Membrane protein induced by beta-amyloid treatment antibody

Mib antibody

Piezo-type mechanosensitive ion channel component 1 antibody

Protein FAM38A antibody

Protein FAM38B antibody

Protein PIEZO1 antibody

General Information

Product

Insulin degrading enzyme Antibody

Verified Applications

ELISA, IP WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Insulin-degrading enzyme protein.

Accession #

GenBank: EAW50092.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI00002289E9

Overview

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Molecular Function

ATPase activity; Ubiquitin Binding; Zinc ion binding; Insulin binding; Hydrolase; Metalloprotease; Protease

Subcellular Location

Cytoplasm; Cell membrane; Secreted

Structure

Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.

Alternative Nomenclature

Abeta-degrading protease antibody

FLJ35968 antibody

Ide antibody

insulin protease antibody

Insulin-degrading enzyme antibody

insulinase antibody

insulysin antibody

General Information

Product

Pin1 Antibody

Description

Peptidyl-prolyl cis-trans isomerase Pin1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.

Accession #

NCBI:  XP_011526370.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western blot

1:500

Protein

Uniprot #

UPI0005D038B7

Overview

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).

Molecular Function

GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Expression

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.

Structure

Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).

Alternative Nomenclature

DOD antibody

DODO, Drosophila, homolog of antibody

FLJ40239 antibody

FLJ77628 antibody

MGC10717 antibody

NIMA interacting 1 antibody

Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody

Pin 1 antibody

PPIase Pin1 antibody

Prolyl isomerase antibody

Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody

Protein NIMA interacting 1 antibody

Rotamase Pin1 antibody

UBL5 antibody

General Information

Product

Pin1 Antibody

Description

Peptidyl-prolyl cis-trans isomerase Pin1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 or 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.

Accession #

NCBI:  XP_011526370.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0005D038B7

Overview

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).

Molecular Function

GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Expression

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.

Structure

Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).

Alternative Nomenclature

DOD antibody

DODO, Drosophila, homolog of antibody

FLJ40239 antibody

FLJ77628 antibody

MGC10717 antibody

NIMA interacting 1 antibody

Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody

Pin 1 antibody

PPIase Pin1 antibody

Prolyl isomerase antibody

Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody

Protein NIMA interacting 1 antibody

Rotamase Pin1 antibody

UBL5 antibody

General Information

Product

Presenilin 1 Antibody

Description

Affinity Purified Presenilin 1 (Alzheimer disease 3) Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Cat, Human, Monkey, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 417-467 on human Presnelin-1 protein.

Accession #

GenBank:  EAW81096.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P49768

Overview

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.

Molecular Function

Hydrolase, Protease

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface

Expression

Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.

Structure

Homodimer

Alternative Nomenclature

AD3 antibody

FAD antibody

Homo Sapiens Clone CC44 Senilin 1 antibody

Presenilin-1 CTF12 antibody

Protein S182 antibody

PS 1 antibody

PSEN1 antibody

S182 antibody

General Information

Product

Presenilin 1 Antibody Affinity Purified

Description

Affinity Purified Presenilin 1 (Alzheimer disease 3) Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 1-50 on Presenilin 1 (Alzheimer disease 3) protein.

Accession #

GenBank:  EAW81096.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P49768

Overview

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.

Molecular Function

Hydrolase, Protease

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface

Expression

Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.

Structure

Homodimer

Alternative Nomenclature

AD3 antibody

FAD antibody

Homo Sapiens Clone CC44 Senilin 1 antibody

Presenilin-1 CTF12 antibody

Protein S182 antibody

PS 1 antibody

PSEN1 antibody

S182 antibody

General Information

Product

MAPK3 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P27361

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

Mitogen-activated protein kinase 3 Antibody MAP kinase 3 Antibody

MAPK 3 Antibody

ERT2 Antibody

Extracellular signal-regulated kinase 1 Antibody ERK-1 Antibody

Insulin-stimulated MAP2 kinase Antibody

MAP kinase isoform p44 Antibody p44-MAPK Antibody Microtubule-associated protein 2 kinase Antibody

p44-ERK1 Antibody

MAPK3 Antibody

ERK1 Antibody

PRKM3 Antibody

General Information

Product

OGT1 Antibody

Description

Affinity Purified O-linked N-acetylglucosamine transferase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human O-linked N-acetylglucosamine transferase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15294

Overview

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.

Molecular Function

Chromatin regulator, Glycosyltransferase, Transferase

Subcellular Location

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Expression

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

Structure

The TPR repeat domain is required for substrate binding and oligomerization.

Alternative Nomenclature

GlcNAc transferase

HRNT1 

O GlcNAc 

O GlcNAc transferase p110 subunit 

O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) 

UDP N acetylglucosamine peptide N

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit