General Information

Product

Neurochondrin Antibody 

Description

NORBIN Antibody

Verified Applications

ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on rat NORBIN protein.

Accession #

GenBank: BAA22938.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1;10,000

ELISA

1;10,000

Western Blot

1:500

Protein

Uniprot #

O35095

Overview

Involved in regulation of neuronal synaptic plasticity;neuron projection development;bone resorption

Subcellular Location

Cell projection, Cytoplasm,somatic regions of neurons

Expression

Expressed in brain and in peripheral nervous system (at protein level). Weakly expressed in neurites.

Structure

Belongs to the neurochondrin family.

Alternative Nomenclature

AU042419 antibody

KIAA0607 antibody

M-Sema F-associating protein of 75 kDa antibody

MGC93259 antibody

MMS10-AE antibody

NCDN antibody

NCDN-1 antibody

NCDN-2 antibody

Neurochondrin 1 antibody

Norbin antibody

Sfap75 antibody

General Information

Product

Lin28 Antibody

Description

Affinity Purified LIN28 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid sequence 1-60 on Lin28A sequence.

Accession #

GenBank: AAH28566.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q9H9Z2

Overview

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Association with translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing mRNA's

Molecular Function

DNA binding, mRNA binding, zinc ion binding, miRNA binding, RNA binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: nucleolar localization observed in 10-15% of nuclei in differentiated byotubes. Shuttles between cytoplasm and nucleus. Localizes to cytoplasmic processing bodies and stress granules)

Expression

Expressed in embryonic stem cells (ES cells), placenta and testis.

Alternative Nomenclature

AL024421 antibody

CSDD1 antibody

FLJ12457 antibody

Lin 28 antibody

Protein lin-28 homolog A antibody

RNA binding protein lin 28 antibody

Tex17 antibody

ZCCHC1 antibody

Zinc finger CCHC domain containing 1 antibody

General Information

Product

Npas4 Antibody Affinity Purified

Description

Affinity Purified NPAS4 protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human Npas4 protein.

Accession #

GenBank: AAI43631.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1;10,000

ELISA

1;10,000

Western Blot

1:500

Protein

Uniprot #

Q8IUM7

Overview

Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Molecular Function

DNA binding;signal transducer activity

Subcellular Location

Nucleus

Expression

Brain.

Alternative Nomenclature

bHLH PAS type transcription factor NXF antibody

bHLHe79 antibody

Class E basic helix-loop-helix protein 79 antibody

HLH-PAS transcription factor NXF antibody

Le PAS antibody

Neuronal PAS4 antibody

NXF antibody

PAS domain-containing protein 10 antibody

PASD10 antibody

General Information

Product

PKC epsilon Antibody

Description

Affinity Purified Protein Kinase C epsilon Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Protein Kinase C epsilon type protein.

Accession #

NCBI:  NP_005391.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02156

Overview

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardio myocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAP, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implication the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaciton with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a rolei n the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). invovled in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be invovled in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin-and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. IS able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines produciton. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Cell Membrane; Nucleus

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative Nomenclature

KPCE_HUMAN antibody

MGC125656 antibody

MGC125657 antibody

nPKC epsilon antibody

nPKC-epsilon antibody

PKCE antibody

Pkcea antibody

PRKCE antibody

Protein kinase C epsilon antibody

General Information

Product

Cytochrome P450 CYP307a1 Antibody Affinity Purified

Description

Affinity Purified Cytochrome P450 CYP307a1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 500-600 on D. melanogaster Spook protein.

Accession #

GenBank: AAQ05973.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9VRM7

Overview

Required for correct development of the embryonic midline glial cells which are necessary for the formation of distinct segmental commissures.

Molecular Function

heme binding, iron ion binding, monooxygenase activity, oxidoreductase activity

Subcellular Location

endoplasmic reticulum membrane, peripheral membrane protein

Alternative Nomenclature

Cytochrome P450 307a1 antibody

307a1 antibody

CG10594 antibody

CYP307A1-Dm antibody

CG10594 antibody

Dmspo antibody

Spo antibody

spo spook antibody

General Information

Product

SorLA Antibody

Description

Affinity Purified Sortilin-related receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 2100-2200 on human SORLA receptor protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O88307

Overview

Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction. May play a role in neural organization, as well as the establishment of embryonic organ systems.

Molecular Function

beta-amyloid binding, low-density lipoprotein particle binding

Subcellular Location

membrane, single-pass type I membrane protein, golgi apparatus, secreted

Expression

Abundant in brain, where it is mainly expressed in adult cerebellum, hippocampal ca regions, dentate gyrus, and to a much lesser extent in the cerebral cortex. Detectable in kidney, skeletal muscle, lung and spleen, but not in the liver.

Alternative Nomenclature

C11orf32 antibody

gp250 antibody

LDLR relative with 11 ligand binding repeats antibody

Low-density lipoprotein receptor relative with 11 ligand-binding repeats antibody

LR11 antibody

LRP9 antibody

Mosaic protein LR11 antibody

SORL1 antibody

SorLA 1 antibody

Sortilin related receptor antibody

Sorting protein related receptor containing LDLR class A repeats antibody

Sorting protein-related receptor containing

General Information

Product

Alpha SNAP Antibody

Description

Affinity Purified alpha-soluble NSF attachment protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 195-245 on human alpha-soluble NSF attachment protein.

Accession #

GenBank: AAC80170.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P54920

Overview

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.

Molecular Function

Soluble NSF attachment protein activity; Syntaxin binding

Subcellular Location

Membrane, peripheral membrane protein

Structure

Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. Found in a complex with VAMP8. Component of a SNARE-like complex.

Alternative Nomenclature

Alpha soluble NSF attachment protein antibody

N ethylmaleimide sensitive factor attachment protein alpha antibody

NAPA antibody

SNAP alpha antibody

SNAPA antibody

General Information

Product

Olig2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Olig2 protein.

Accession #

GenBank:  AAH36245.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry

1:100

Western Blot

1:500

Protein

Uniprot #

Q13516

Overview

Necessary for oligodendrocyte and motor neuron specification in the spinal cord.  In the hindbrain it is  needed in the development of somatic neurons.

Molecular Function

Developmental protein, DNA binding

Subcellular Location

Nucleus; Cytoplasm

Expression

Is expressed in the brain in oligodendrocytes.  Is expressed strongly in oligodendrogliomas with weaker expression in astrocytomas

Structure

Interacts with NKX2-3

Alternative Nomenclature

Basic domain helix loop helix protein class B 1 antibody

BHLHB antibody

bHLHB1 antibody

bHLHe19 antibody

Class B basic helix loop helix protein 1 antibody

class E basic helix loop helix protein 19 antibody

Human protein kinase C binding protein RACK17 antibody

Olig2 antibody

Oligo2 antibody

Oligodendrocyte lineage transcription factor 2 antibody

Oligodendrocyte specific bHLH transcription factor 2 antibody

PRKCBP2 antibody

Protein kinase C binding protein 2 antibody

Protein kinase C binding protein RACK17 antibody

RACK17 antibody

General Information

Product

Olig2 Antibody

Description

Affinity Purified OLIG2 protein Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to Human Olig2 amino acids 136-156. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to a carrier protein using hetero-bifunctional cross linker for immunogen preparation.

Sequence:

PYAHGPSVRKLSKIATLLLAR

Accession #

GenBank:  AAH36245.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13516

Overview

Necessary for oligodendrocyte and motor neuron specification in the spinal cord.  In the hindbrain it is  needed in the development of somatic neurons.

Molecular Function

Developmental protein, DNA binding

Subcellular Location

Nucleus; Cytoplasm

Expression

Is expressed in the brain in oligodendrocytes.  Is expressed strongly in oligodendrogliomas with weaker expression in astrocytomas

Structure

Interacts with NKX2-3

Alternative Nomenclature

Basic domain helix loop helix protein class B 1 antibody

BHLHB antibody

bHLHB1 antibody

bHLHe19 antibody

Class B basic helix loop helix protein 1 antibody

class E basic helix loop helix protein 19 antibody

Human protein kinase C binding protein RACK17 antibody

Olig2 antibody

Oligo2 antibody

Oligodendrocyte lineage transcription factor 2 antibody

Oligodendrocyte specific bHLH transcription factor 2 antibody

PRKCBP2 antibody

Protein kinase C binding protein 2 antibody

Protein kinase C binding protein RACK17 antibody

RACK17 antibody

General Information

Product

PAX5 Antibody

Description

Affinity Purified Paired box protein PAX-5 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human PAX5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q02548

Overview

May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.

Molecular Function

Developmental protein, RNA polymerase II core promoter proximal region sequence-specific DNA binding

Subcellular Location

Nucleus

Expression

Expressed at early B-cell differentiation, in the developing CNS and in adult testis.

Structure

Interacts with DAXX (By similarity). Binds DNA as a monomer. Binds TLE4. Interacts with ETS1, altering its DNA-binding properties.

Alternative Nomenclature

•                               B cell lineage specific activator antibody
•                               BSAP antibody

•                               EBB-1 antibody
•                               KLP antibody
•                               Paired box 5 antibody
•                               PAX 5 antibody
•                               Transcription factor PAX 5 antibody