General Information

Product

IL12 p40 Antibody

Description

Affinity Purified Interleukin-12 subunit beta precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 280-335 on rat Interleukin-12 subunit beta protein.

Accession #

NCBI:  NP_072133.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

ffinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9R278

Overview

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Molecular Function

Cytokine receptor activity, protein heterodimerization activity, interleukin-12 alpha subunit binding

Alternative Nomenclature

40 kD Subunit antibody

CLMF 2 antibody

CLMF antibody

Cytotoxic lymphocyte maturation factor 2 antibody

Cytotoxic lymphocyte maturation factor 2 p40 antibody

HGNC:5970 antibody

IL 12 subunit p40 antibody

IL 12B antibody

Interleukin 12 beta chain antibody

Interleukin 12 p40 antibody

Natural killer cell stimulatory factor 2 antibody

NKSF 2 antibody

NKSF antibody

p40 antibody

TcMF antibody

TSF antibody

General Information

Product

CD80 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on rat CD80 protein.

Accession #

GenBank: AAC02262.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

O55202

Overview

Integral component of membrane

Subcellular Location

Membrane

Structure

Disulfide bonds

Alternative Nomenclature

Activation B7 1 antigen antibody

B7 antibody

B7-1 antibody

BB1 antibody

CD 80 antibody

CD28 antigen ligand 1 antibody

CD28 antigen ligand 1 B7 1 antigen antibody

CD28LG antibody

CD80 antibody

Costimulatory factor CD80 antibody

costimulatory molecule variant IgV-CD80 antibody

CTLA 4 counter receptor B7.1 antibody

LAB7 antibody

T-lymphocyte activation antigen CD80 antibody

General Information

Product

Topoisomerase II alpha Antibody

Description

Affinity Purified DNA topoisomerase (ATP-hydrolysing) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

Accession #

GenBank: CAA09762.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11388

Overview

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Molecular Function

Isomerase, Topoisomerase, ATP binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity.

Alternative Nomenclature

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

TOP2 antibody

TOP2A antibody

Topoisomerase DNA II alpha 170kDa antibody

TP2A antibody

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

General Information

Product

TLR9 Antibody

Description

Affinity Purified Toll-like receptor 9 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-460 on human TLR9 protein.

Accession #

GenBank: AAQ89443.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57-0.76 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q9NR96

Overview

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides.

Molecular Function

Receptor, Interleukin-1 receptor binding, siRNA binding

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein, Endosome, Lysosome, Cytoplasmic vesicle

Expression

Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

Structure

Interacts with MYD88 via their respective TIR domains (By similarity). Interacts (via transmembrane domain) with UNC93B1 (By similarity). Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses.

Alternative Nomenclature

CD 289 antibody

TLR 9 antibody

Toll like receptor 9 isoform A precursor antibody

Toll like receptor 9 isoform B antibody

General Information

Product

TLR7 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 7 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Mouse, Pig

Immunogen

Synthetic peptide taken within amino acid region 175-225 on human TLR7 protein.

Accession #

NCBI:  NP_057646.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q9NYK1

Overview

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor, double-stranded RNA bindng, single-stranded RNA binding

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein, Endosome, Lysosome, Cytoplasmic vesicle

Expression

Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

Structure

Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1 (By similarity).

Alternative Nomenclature

PRO285 antibody

TLR 7 antibody

Toll like receptor 7 antibody

UNQ248 antibody

General Information

Product

TLR6 Antibody

Description

Affinity Purified Toll-like receptor 6 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Mouse, Rat, Pig

Immunogen

Synthetic peptide taken within amino acid region 195-225 on human TLR6 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.78-0.80 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q9Y2C9

Overview

Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.

Molecular Function

Receptor, diacyl lipopeptide binding, transmembrane signaling receptor activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein, Cytoplasmic vesicle >  phagosome membrane; Single-pass type I membrane protein

Expression

Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

Structure

Binds MYD88 via their respective TIR domains. Heterodimer with TLR2 via their respective extracellular domains (By similarity). Interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers inflammatory response. LYN kinase activity facilitates TLR4-TLR6 heterodimerization and signal initation.

Alternative Nomenclature

CD286 antibody

TLR 6 antibody

Toll like receptor 6 precursor antibody

General Information

Product

TLR5 Antibody

Description

Affinity Purified Toll-like receptor 5 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 195-225 on human TLR5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

O60602

Overview

Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Molecular Function

Receptor, interleukin-1 receptor binding, transmembrane signalling receptor activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.

Structure

Binds MYD88 via their respective TIR domains. Homodimer both in the absence and presence of ligand.

Alternative Nomenclature

SLEB1 antibody

TIL3 antibody

Toll like receptor 5 antibody

Toll/interleukin 1 receptor like protein 3 antibody

General Information

Product

TLR4 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 970-1000 on human TLR4 protein.

Accession #

GenBank: AAF05316.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

O00206

Overview

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+.

Molecular Function

Receptor, lipopolysaccharide binding, transmembrane signaling receptor activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Structure

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via the

Alternative Nomenclature

ARMD10 antibody

CD284 antibody

Homolog of Drosophila toll antibody

hToll antibody

TLR 4 antibody

Toll (Drosophila) homolog antibody

TOLL antibody

Toll Endotoxin Hyporesponsiveness antibody

Toll like receptor 4 antibody

General Information

Product

TLR3 Antibody

Description

Affinity Purified Toll-like receptor 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-700 on human TLR3 protein.

Accession #

GenBank: AAH96333.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.77 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O15455

Overview

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection.

Molecular Function

Receptor, double-stranded RNA binding, transmembrane signaling receptor activity

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein, Endosome membrane

Expression

Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

Structure

Monomer and homodimer; dimerization is triggered by ligand-binding, the signaling unit is composed of one ds-RNA of around 40 bp and two TLR3 molecules, and lateral clustering of signaling units along the length of the ds-RNA ligand is required for TLR3 signal transduction. Interacts (via transmembrane domain) with UNC93B1; the interaction is required for transport from the ER to the endosomes.

Alternative Nomenclature

CD283 antibody

CD283 antigen antibody

IIAE2 antibody

TLR 3 antibody

Toll Like Receptor 3 antibody

General Information

Product

TLR12 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 12 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 440-470 from mouse TLR12 protein.

Accession #

NCBI:  NP_991392.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q6QNU9

Overview

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Macrophages, liver, kidney and bladder epithelial cells.

Structure

Binds MYD88 via their respective TIR domains.

Alternative Nomenclature

Toll-like receptor 12