General Information

Product

U2AF59 Antibody

Description

Affinity Purified Splicing factor U2AF 59 kDa subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cerevisiae (yeast)

Immunogen

Synthetic peptide taken within amino acid region 480-517 on yeast U2AF59 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.13 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

P36629

Overview

Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.

Molecular Function

mRNA binding, snoRNA binding, nucleotide binding

Subcellular Location

Nucleus

Structure

Forms a heterodimer with the U2AF small subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with wat1/pop3.

Alternative Nomenclature

S. pombe splicing factor U2 59 kDa subunit

Schzosacharomyces pombe splicing factor U2 auxiliary factor

U2 auxiliary factor 59 kDa subunit

U2 snRNP auxiliary factor large subunit

mis11

prp2

General Information

Product

TIPIN Antibody

Description

Affinity Purified Timeless-interacting protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 240-270 on mouse TIPIN protein.

Accession #

GenBank: DAA01365.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.96-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BVW5

Overview

Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress.

Subcellular Location

Cytoplasm, Nucleus

Structure

Interacts with TIMELESS, which impairs TIMELESS self-association. Interacts with RPA2, PRDX2, MCM6 and MCM7.

Alternative Nomenclature

FLJ20516 antibody

TIMELESS interacting protein antibody

TIPIN antibody

General Information

Product

Quiescine Q6 Antibody

Description

Affinity Purified Sulfhydryl oxidase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on human QSOX1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O00391

Overview

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.  May contribute to disulfide bond formation in a variety of secreted proteins.

Molecular Function

Oxidoreductase, flavin-linked sulfhydryl oxidase activity, protein disulfide isomerase activity

Subcellular Location

Isoform 1: Golgi apparatus membrane; Single plass membrane protein Isoform 2: Secreted > extracellular space

Expression

Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney.

Structure

Monomer

Alternative Nomenclature

FLJ34858 antibody

hQSOX antibody

Human quiescin (Q6) mRNA, partial cds antibody

Q6 antibody

QSCN6 antibody

QSOX1 antibody

Quiescin Q6 antibody

Quiescin Q6 sulfhydryl oxidase 1 antibody

Skin sulfhydryl oxidase antibody

Sox antibody

Sulfhydryl oxidase 1 antibody

General Information

Product

RASGRF2 Antibody

Description

Affinity Purified Ras-specific guanine nucleotide-releasing factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 1100-1200 on human RASGRF2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O14827

Overview

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP.  Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation.

Molecular Function

Guanine-nucleotide releasing factor, Rho guayl-nucleotide exchange factor activity

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Endoplasmic reticulum membrane; Peripheral membrane protein

Expression

Widely expressed with higher expression in the brain, followed by heart, lung, pancreas and kidney.  Detected in placenta.  Expressed in brain and lung.

Structure

Homooligomer and heterooligomer with RASGRF1.  Interacts with Ras and RAC1.  Interacts in a calcium-dependent manner with calmodulin.

Alternative Nomenclature

GRF2 antibody

Ras guanine nucleotide exchange factor 2 antibody

Ras-GRF2 antibody

General Information

Product

ergo-1 Antibody 

Description

Affinity Purified Endogenous-RNAi deficient arGOnaute Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 190-240 on C.elegans Endogenous-RNAi deficient arGOnaute.

Accession #

NCBI:  NP_503362.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O61931

Overview

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).

Molecular Function

Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.

Subcellular Location

Cytoplasm

Structure

Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Alternative Nomenclature

Endogenous RNAi deficient argonaute antibody

Ergo 1 antibody

Hypothetical protein R09A1.1 antibody

General Information

Product

ergo-1 Antibody

Description

Affinity Purified Endogenous-RNAi deficient arGOnaute antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 790-840 on C.elegans Endogenous-RNAi deficient arGOnaute protein.

Accession #

NCBI:  NP_503362.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O61931

Overview

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).

Molecular Function

Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.

Subcellular Location

Cytoplasm

Structure

Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Alternative Nomenclature

Endogenous RNAi deficient argonaute antibody

Ergo 1 antibody

Hypothetical protein R09A1.1 antibody

General Information

Product

TRF2 Antibody Affinity Purified

Description

Affinity Purified Telomeric Repeat-binding Factor 2 Antibody Mid-Region

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Telomeric repeat-binding factor 2 protein.

Accession #

GenBank: AAB81135.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

Q15554

Overview

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Molecular Function

Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding

Subcellular Location

Nucleus; Chromosome > telomere

Expression

Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.

Structure

Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.

Alternative Nomenclature

Telomeric DNA binding protein antibody

Telomeric repeat binding factor 2 antibody

TERF 2 antibody

TRBF 2 antibody

TRF 2 antibody

TTAGGG repeat-binding factor 2 antibody

General Information

Product

TRF2 Antibody

Description

Affinity Purified Telomeric Repeat-binding Factor 2 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 80-130 on human Telomeric repeat-binding factor 2 protein.

Accession #

GenBank: AAB81135.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

Q15554

Overview

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Molecular Function

Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding

Subcellular Location

Nucleus; Chromosome > telomere

Expression

Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.

Structure

Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.

Alternative Nomenclature

Telomeric DNA binding protein antibody

Telomeric repeat binding factor 2 antibody

TERF 2 antibody

TRBF 2 antibody

TRF 2 antibody

TTAGGG repeat-binding factor 2 antibody

General Information

Product

TRF2 Antibody Affinity Purified

Description

Affinity Purified Telomeric Repeat-binding Factor 2 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 360-410 on human Telomeric repeat-binding factor 2 protein.

Accession #

GenBank: AAB81135.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

Q15554

Overview

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Molecular Function

Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding

Subcellular Location

Nucleus; Chromosome > telomere

Expression

Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.

Structure

Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.

Alternative Nomenclature

Telomeric DNA binding protein antibody

Telomeric repeat binding factor 2 antibody

TERF 2 antibody

TRBF 2 antibody

TRF 2 antibody

TTAGGG repeat-binding factor 2 antibody

General Information

Product

Cyclin D1 Antibody

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 1-50 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody