General Information

Product

Aurora A Antibody

Description

Affinity Purified Aurora Kinase A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-397 on human Aurora Kinase A protein.

Accession #

NCBI:  NP_695208

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q3MHU2

Overview

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes.

Molecular Function

ATP binding

histone serine kinase activity

protein heterodimerization activity

protein kinase activity

protein kinase binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

ubiquitin protein ligase binding

Subcellular Location

centrosome 

spindle pole 

cilium basal body

centriole

Expression

Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK antibody

ARK1 antibody

AURA antibody

Aurora 2 antibody

Aurora A antibody

Aurora kinase A antibody

Aurora-related kinase 1 antibody

Aurora/IPL1 like kinase antibody

AURORA2 antibody

Breast tumor-amplified kinase antibody

BTAK antibody

hARK1 antibody

IAK antibody

IPL1 related kinase antibody

PPP1R47 antibody

Protein phosphatase 1, regulatory subunit 47 antibody

Serine/threonine kinase 15 antibody

Serine/threonine kinase 6 antibody

Serine/threonine-protein kinase aurora-A antibody

STK15 antibody

STK6 antibody

STK7 antibody

General Information

Product

Aurora B Antibody

Description

Aurora Kinase B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 300-344 on human Aurora Kinase B protein.

Accession #

NCBI:  NP_004208.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q96GD4

Overview

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.

Molecular Function

ATP binding

histone serine kinase activity

kinase binding

metal ion binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

Subcellular Location

Spindle, Nucleus

Expression

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK2 antibody

AIM1 antibody

ARK2 antibody

AurB antibody

AURKB antibody

Aurora 1 antibody

Aurora and Ipl1 like midbody associated protein 1 antibody

Aurora kinase B antibody

Aurora related kinase 2 antibody

Aurora- and Ipl1-like midbody-associated protein 1 antibody

Aurora-B antibody

Aurora/IPL1 related kinase 2 antibody

IPL1 antibody

PPP1R48 antibody

Protein phosphatase 1 regulatory subunit 48 antibody

Serine/threonine-protein kinase 12 antibody

Serine/threonine-protein kinase aurora-B antibody

STK1 antibody

STK12 antibody

STK5 antibody

General Information

Product

LRRK1 Antibody

Description

Affinity Purified Leucine-rich repeat serine/threonine-protein kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1950-2015 on human Leucine-rich repeat serine/threonine-protein kinase 1  protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q38SD2

Overview

ATP + a protein = ADP + a phosphoprotein.

Molecular Function

ATP binding

GTP binding

identical protein binding

metal ion binding

protein serine/threonine kinase activity

signal transducer activity

Subcellular Location

Cytoplasm

Expression

Homodimer.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.

Alternative Nomenclature

KIAA1790 antibody

Leucine rich repeat serine/threonine protein kinase 1 antibody

Lrrk1 antibody

RIPK6 antibody

Roco1 antibody

General Information

Product

Phospho-STAT6 Antibody

Description

Affinity Purified Phospho-specific Signal transducer and activator of transcription 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 610-660 on human Phospho-specific Signal transducer and activator of transcription 6  protein. Phosphorylated Tyrosine 641 residue.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P42226

Overview

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Subcellular Location

Nucleus

Structure

Belongs to the transcription factor STAT family.

Alternative Nomenclature

IL4 STAT antibody

Interleukin 4 Induced antibody

Interleukin 4 Induced Transcription Factor IL4 STAT antibody

Signal transducer and activator of transcription 6 antibody

Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody

Signal transducer and activator of transcription 6, interleukin 4 induced antibody

STAT 6 antibody

STAT interleukin4 induced antibody

Stat6 antibody

STAT6B antibody

STAT6C antibody

Transcription factor IL 4 STAT antibody

General Information

Product

B19 Antibody

Description

Affinity Purified Hormonally Up-regulated Neu tumor-associated Kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-714 on mouse Hormonally Up-regulated Neu tumor-associated Kinase.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

O88866

Overview

ATP binding serine/threonine kinase activity

Subcellular Location

Nucleus, Cytoplasm

Expression

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Structure

Hormonally Up-regulated Neu tumor-associated Kinase

Alternative Nomenclature

B19 antibody

Bstk1 antibody

Hormonally up-regulated neu tumor-associated kinase antibody

HUNK antibody

MAKV antibody,

Serine/threonine-protein kinase MAK-V antibody

General Information

Product

DAP Kinase 1 Antibody

Description

Affinity Purified Death-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1370-1420 on human Death-associated protein kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P53355

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy.

Molecular Function

ATP binding

calmodulin binding

calmodulin-dependent protein kinase activity

GTP binding

identical protein binding

protein kinase activity

protein serine/threonine kinase activity

syntaxin-1 binding

Subcellular Location

Cytoskeleton

Expression

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK1 antibody

DAP K1 antibody

DAP kinase 1 antibody

DAPK antibody

DAPK1 antibody

Death Associated Protein Kinase 1 antibody

General Information

Product

DAPK2 Antibody

Description

Affinity Purified Death-associated protein kinase 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide take within amino acid region 300-350 on mouse Death-associated protein kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8VDF3

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Capable of regulating both type I apoptotic and type II autophagic cell death signals.

Molecular Function

ATP binding

calmodulin binding

identical protein binding

protein serine/threonine kinase activity

Subcellular Location

Cytoplasm

autophagosome lumen

Expression

Expressed in peritubular interstitial cells of the renal cortex (PubMed:24906443). Isoform 1 is found in the adult brain while isoform 2 is expressed in brains of embryos and young mice (at protein level) (PubMed:21408167).

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK2 antibody

DAP kinase 2 antibody

DAP kinase related protein 1 antibody

DAP kinase related protein 1 beta isoform antibody

DAP-kinase-related protein 1 antibody

DAPK2 antibody

Death associated protein kinase 2 antibody

DRP 1 antibody

General Information

Product

ZIP Kinase Antibody

Description

Affinity Purified Death-associated protein kinase 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region on 400-448 on mouse Death-associated protein kinase 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

O54784

Overview

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization.

Molecular Function

ATP binding

identical protein binding

kinase activity

leucine zipper domain binding

protein C-terminus binding

protein homodimerization activity

protein serine/threonine kinase activity

Rho GTPase binding

Subcellular Location

Cytoskeleton, Nucleus

Expression

Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.

Alternative Nomenclature

DAP kinase 3 antibody

DAP like kinase antibody

DAPK3 antibody

Death associated kinase 3 antibody

Death associated protein kinase 3 antibody

Dlk antibody

MYPT1 kinase antibody

ZIP antibody

ZIP kinase antibody

ZIP kinase isoform antibody

ZIPK antibody

zipper-interacting protein kinase antibody