General Information

Product

NEDD4 Antibody

Description

Affinity Purified E3 ubiquitin-protein ligase NEDD4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 840-880 on rat Nedd4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q62940

Overview

E3 ubiquitin-protein ligase;Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding

Molecular Function

Ligase

Subcellular Location

Cell membrane, Cytoplasm, Membrane

Expression

Ubiquitously expressed. Expression is highest in lung, kidney and brain

Alternative Nomenclature

Cell proliferation-inducing gene 53 protein antibody

E3 ubiquitin protein ligase Nedd4 antibody

KIAA0093 antibody

NEDD 4 antibody

Neural precursor cell expressed developmentally down regulated 4 antibody

General Information

Product

Nogo Antibody

Description

Affinity Purified Reticulon-4 isoform A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1100-1200 on Nogo AS protein.

Accession #

NCBI:  NP_065393.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9NQC3

Overview

Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching.  Regulates neurite fasciculation, branching and extension in the developing nervous system.

Molecular Function

poly(A) RNA binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein

Expression

Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle.   Isoform 2 is widely expressed except for the liver.  Isoform 3 is expressed in brain, skeletal muscle and adipocytes.  Isoform 4 is testis-specific.

Structure

Binds to RTN4R, interacts with Bcl-xl and Bcl-2.  Isoform 2 binds to NGBR and RTN3.  Isoform 2 and Isoform 3 interact with BACE1 and BACE2, interacts with RTN4IP1 and ATL1.

Alternative Nomenclature

1110020G17Rik antibody

AA407876 antibody

AA409940 antibody

AA960376 antibody

ASY antibody

C130026I10Rik antibody

Foocen antibody

Glut4 vesicle 20 kDa protein antibody

Human NogoA antibody

Kiaa0886 antibody

KIAA4153 antibody

MGC116054 antibody

MGC139261 antibody

mKIAA0886 antibody

mKIAA4153 antibody

My043 protein antibody

Nbla00271 antibody

Nbla10545 antibody

Neurite growth inhibitor 220 antibody

Neuroendocrine-specific protein antibody

NI-250 antibody

Nogo A antibody

NOGO antibody

Nogo B antibody

Nogo C antibody

NSP antibody

NSP CL antibody

rat N antibody

Reticulon 4 antibody

Reticulon 5 antibody

RTN X antibody

RTN4 A antibody

RTN4-B1 antibody

Vp20 antibody

General Information

Product

Netrin 1 Antibody Affinity Purified

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human Netrin-1 protein.

Accession #

NCBI: NP_004813.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O95631

Overview

Netrins control guidance of CNS commissural axons and peripheral motor axons; serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis;Involved in tumorigenesis by regulating apoptosis.

Subcellular Location

Extracellular matrix, Secreted

Expression

highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).

Alternative Nomenclature

epididymis tissue protein Li 131P antibody

Netrin 1, mouse, homolog of antibody

Netrin 1-like antibody

Ntn 1 antibody

Unc6 antibody

General Information

Product

Nurr1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to amino acid region 566-591. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker. 

Sequence:

CTQGLQRIFYLKLEDLVPPPAIIDKL

Accession #

GenBank: BAA75666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P43354

Overview

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development.

Molecular Function

Receptor

Subcellular Location

Cytoplasm, Nucleus

Expression

Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.

Structure

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Alternative Nomenclature

HZF 3 antibody

Immediate-early response protein NOT antibody

NGFI B/nur77 beta type transcription factor homolog antibody

NOT antibody

NR4A2 antibody

Nur related protein 1 homolog antibody

Nurr 1 antibody

Orphan nuclear receptor NR4A2 antibody

RNR1 antibody

T cell nuclear receptor NOT antibody

TINUR antibody

Transcriptionally inducible nuclear receptor antibody

General Information

Product

PAR3 Antibody

Description

Affinity Purified Proteinase-activated receptor 3 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on mouse Proteinase-activated receptor 3 protein.

Accession #

NCBI: NP_032001.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:12,000

ELISA

1:12,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:5,000

Protein

Uniprot #

O88634

Overview

Functions as a receptor for activated thrombin or trypsin coupled to G-proteins that stimulate phosphoinositide hydrolysis.  May also function in contributing to the activation of platelets.

Molecular Function

G-protein coupled receptor, Receptor, Transducer, thrombin receptor activity

Alternative Nomenclature

agouti signaling protein antibody

ASIP antibody

Atypical PKC isotype specific interacting protein antibody

Baz antibody

bazooka antibody

Coagulation factor II receptor-like 2 antibody

CTCL tumor antigen se2-5 antibody

F2rl2 antibody

PAR-3 antibody

PAR3-alpha antibody

PARD-3 antibody

Partitioning-defective protein 3, C. elegans, homolog of antibody

SE2-5L16 antibody

SE2-5LT1 antibody

SE2-5T2 antibody

Thrombin receptor-like 2 antibody

General Information

Product

Phospho Ephrin receptor A4 Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Ephrin type-A receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 570-630 on human EPHA R4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q03137

Overview

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via

Alternative Nomenclature

Cek 8 antibody

EK8 antibody

eph receptor a4 antibody

EPH-like kinase 8 antibody

EphA4 antibody

Ephrin receptor Eph A4 antibody

hEK8 antibody

Receptor protein tyrosine kinase HEK 8 antibody

Sek 1 antibody

SEK antibody

TYRO 1 protein tyrosine kinase antibody

Tyrosine protein kinase receptor SEK antibody

General Information

Product

Phospho Survivin Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Baculoviral IAP repeat-containing protein 5 isoform 2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on human Survivin protein.

Accession #

NCBI:  NP_001012270.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-1.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O15392

Overview

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Molecular Function

Protease inhibitor, Repressor, Thiol protease inhibitor

Subcellular Location

Cytoplasm, Nucleus, Chromsome, Centromere, Cytoskeleton, Spindle, Kinetochore, Midbody

Expression

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Structure

Monomer or Homodimer.

Alternative Nomenclature

API4 antibody

Apoptosis Inhibitor 4 antibody

Apoptosis inhibitor survivin antibody

Baculoviral IAP repeat-containing protein 5 antibody

BIRC5 antibody

EPR 1 antibody

IAP4 antibody

Survivin variant 3 alpha antibody

SVV antibody

TIAP antibody

General Information

Product

REST / NRSF Antibody Affinity Purified

Description

Affinity Purified RE1-silencing transcription factor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1097 on human REST protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.54 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13127

Overview

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes.

Molecular Function

Repressor, Chromatin binding, Core promoter binding

Subcellular Location

Nucleus

Expression

Ubiquitous, expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovaries.

Structure

Interacts with SIN3A, SIN3B and RCOR1.  Interacts with CDYL.

Alternative Nomenclature

Neural Restrictive Silencer Factor antibody

NRSF antibody

RE1 silencing transcription factor antibody

Repressor binding to the X2 box antibody

REST antibody

X2 box repressor antibody

XBR antibody

General Information

Product

ROR1 Antibody

Description

Affinity Purified Receptor tyrosine kinase-like orphan receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to C terminal amino acids 900-918 on Human ROR1 protein. Peptide was coupled to a carrier protein using heterobifunctional cross linker.

Sequence:

TVKGNKSQKPYKIDSKQAS

Accession #

GenBank:  EAX06557.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0000EE3178

Overview

Tyrosine-protein kinase receptor whose role is not yet clear.

Molecular Function

ATP binding; transmembrane receptor protein Tyrosine kinase activity; Wnt-protein binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.

Structure

Contains 1 FZ (frizzled) domain; Contains 1 Ig-like C2-type (immunoglobulin-like) domain; Contains 1 Kringle domain; Contains 1 Protein kinase domain

Alternative Nomenclature

dJ537F10.1 antibody

MGC99659 antibody

Neurotrophic tyrosine kinase antibody

Receptor tyrosine kinase like orphan receptor 1 antibody

receptor-related 1 antibody

RGD1559469 antibody

RP11 24J23.1 antibody

Tyrosine kinase like orphan receptor 1 antibody

General Information

Product

RUNX1 / AML1 Antibody Affinity Purified

Description

Affinity Purified RUNX1 Protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-80 on human RUNX1 protein.

Accession #

GenBank: AAI36381.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q01196

Overview

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis.

Molecular Function

ATP binding, DNA binding, calcium ion binding

Subcellular Location

Nucleus

Expression

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Structure

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize.

Alternative Nomenclature

Acute myeloid leukemia 1 protein antibody

alpha subunit core binding factor antibody

AML1 EVI 1 fusion protein antibody

Aml1 oncogene antibody

AMLCR 1 antibody

CBF alpha 2 antibody

Core binding factor runt domain alpha subunit 2 antibody

Core-binding factor subunit alpha-2 antibody

EVI1 antibody

HGNC antibody

Oncogene AML 1 antibody

PEBP2 alpha B antibody

Polyomavirus enhancer binding protein 2 alpha B subunit antibody

Run1 antibody

Runt-related transcription factor 1 antibody

SL3/AKV core binding factor alpha B subunit antibody