General Information

Product

REST / NRSF Antibody Affinity Purified

Description

Affinity Purified RE1-silencing transcription factor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1097 on human REST protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.54 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13127

Overview

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes.

Molecular Function

Repressor, Chromatin binding, Core promoter binding

Subcellular Location

Nucleus

Expression

Ubiquitous, expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovaries.

Structure

Interacts with SIN3A, SIN3B and RCOR1.  Interacts with CDYL.

Alternative Nomenclature

Neural Restrictive Silencer Factor antibody

NRSF antibody

RE1 silencing transcription factor antibody

Repressor binding to the X2 box antibody

REST antibody

X2 box repressor antibody

XBR antibody

General Information

Product

RUNX1 / AML1 Antibody Affinity Purified

Description

Affinity Purified RUNX1 Protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-80 on human RUNX1 protein.

Accession #

GenBank: AAI36381.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q01196

Overview

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis.

Molecular Function

ATP binding, DNA binding, calcium ion binding

Subcellular Location

Nucleus

Expression

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Structure

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize.

Alternative Nomenclature

Acute myeloid leukemia 1 protein antibody

alpha subunit core binding factor antibody

AML1 EVI 1 fusion protein antibody

Aml1 oncogene antibody

AMLCR 1 antibody

CBF alpha 2 antibody

Core binding factor runt domain alpha subunit 2 antibody

Core-binding factor subunit alpha-2 antibody

EVI1 antibody

HGNC antibody

Oncogene AML 1 antibody

PEBP2 alpha B antibody

Polyomavirus enhancer binding protein 2 alpha B subunit antibody

Run1 antibody

Runt-related transcription factor 1 antibody

SL3/AKV core binding factor alpha B subunit antibody

General Information

Product

RUNX2 Antibody

Description

Affinity Purified Runt-related transcription factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda

Immunogen

Synthetic peptide taken within amino acid region 10-50 on human RUNX2 protein.

Accession #

NCBI:  XP_001100855.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0000D9AD12

Overview

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.

Molecular Function

DNA-binding; Transcription Regulation

Subcellular Location

Nucleus

Structure

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with HIVEP3. Interacts with IFI204. I

Alternative Nomenclature

Acute myeloid leukemia 3 protein antibody

Alpha subunit 1 antibody

AML3 antibody

CBF alpha 1 antibody

CBFA1 antibody

CCD1 antibody

Cleidocranial dysplasia 1 antibody

Core binding factor runt domain alpha subunit 1 antibody

Core binding factor subunit alpha 1 antibody

Oncogene AML 3 antibody

OSF 2 antibody

Osteoblast specific transcription factor 2 antibody

PEBP2 alpha A antibody

PEBP2A1 antibody

PEBP2A2 antibody

Polyomavirus enhancer-binding protein 2 alpha A subunit antibody

Runt domain antibody

Runt related transcription factor 2 antibody SL3 3 enhancer factor 1 alpha A subunit antibody

General Information

Product

SFRP2 Antibody 

Description

Affinity Purified Secreted frizzled-related protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 225-295 on human SFRP2.

Accession #

GenBank: AAH08666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96HF1

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Molecular Function

Endopeptidase activator activity, integrin binding, PDZ domain binding, Wnt-activated receptor activity, fibronectin binding, receptor agonist activity, Wnt-protein binding

Subcellular Location

Secreted

Expression

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Alternative Nomenclature

AI851596 antibody
Frizzled related protein 2 antibody
FRP2 antibody
SARP1 antibody
SDF5 antibody
Secreted apoptosis related protein 1 antibody
Secreted frizzled-related protein 2 antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 340-390 on human SIRT7 protein.

Accession #

GenBank: AAH17305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NRC8

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody

General Information

Product

SREBP2 Antibody

Description

Affinity Purified Sterol regulatory element binding protein-2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.

Accession #

GenBank: AAA50746.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q12772

Overview

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

Molecular Function

Activator, E-box binding, protein C-terminus binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein, Golgi apparatus membrane; Multi-pass membrane protein, Cytoplasmic vesicle > COPII-coated vesicle membrane; Multi-pass membrane protein

Expression

Ubiquitously expressed in adult and fetal tissues.

Structure

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF13.

Alternative Nomenclature

AI608257 antibody

bHLHd2 antibody

Class D basic helix-loop-helix protein 2 antibody

Processed sterol regulatory element-binding protein 2 antibody

SREBF 2 antibody

SREBP2 antibody

Sterol regulatory element-binding transcription factor 2 antibody

General Information

Product

ETS1 associated protein II Antibody

Description

Affinity Purified tyrosyl-DNA phosphodiesterase 2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-360 on human Tyrosyl-DNA phosphodiesterase protein.

Accession #

NCBI:  NP_057698.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

O95551

Overview

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue.

Molecular Function

5'-tyrosyl-DNA phosphodieseterase activity, magnesium ion binding, manganese ion binding, nuclease activity, transcription corepressor activity, single-stranded DNA binding

Subcellular Location

nucleus, PML body, cytoplasm

Expression

Widely expressed

Alternative Nomenclature

5'-tyrosyl-DNA phosphodiesterase antibody

AD 022 antibody

dJ30M3.3 antibody

EAP 2 antibody

ETS 1 associated protein 2 antibody

RP1-30M3.3 antibody

TRAF and TNF receptor associated protein antibody

TTRAP antibody

Tyr DNA phosphodiesterase 2 antibody

General Information

Product

p63 Antibody

Description

Affinity Purified Tumor protein 63 isoform 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human p63 protein.

Accession #

NCBI:  NP_001108454

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H3D4

Overview

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes.

Molecular Function

Activator, Developmental protein, chromatin binding

Subcellular Location

Nucleus

Expression

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. 

Structure

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Alternative Nomenclature

AIS antibody

Amplified in squamous cell carcinoma antibody

B(p51A) antibody

B(p51B) antibody

Chronic ulcerative stomatitis protein antibody

CUSP antibody

DN p63 alpha 1 antibody

EEC3 antibody

Keratinocyte transcription factor antibody

KET antibody

LMS antibody

MGC115972 antibody

MGC192897 antibody

NBP antibody

OFC8 antibody

p40 antibody

p51 antibody

P51/P63 antibody

p53CP antibody

p63 antibody

P73 antibody

p73H antibody

p73L antibody

RHS antibody

SHFM4 antibody

TAp63alpha antibody

TP53CP antibody

TP53L antibody

TP63 antibody

TP73 antibody

Transformation related protein 63 antibody

Trp53rp1 antibody

Trp63 antibody

Tumor protein 63 antibody

Tumor protein p53-competing protein antibody

Tumor protein p63 deltaN isoform delta antibody

Tumor protein p73 antibody

General Information

Product

Protein Vestigial Antibody Affinity Purified

Description

Affinity Purified Vestigial Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 200-250 on D. melanogaster Vestigial protein.

Accession #

NCBI:  NP_523723.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q26366

Overview

Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.

Molecular Function

DNA binding; Protein C-terminus binding

Subcellular Location

Nucleus

Expression

Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.

Alternative Nomenclature

VG

VSTGL

VG21

DMEL/CG3830

CCG3830

General Information

Product

WWOX2 Antibody Affinity Purified

Description

Affinity Purified Abberant WW domain-containing Oxidoreductase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on WWOX2 protein.

Accession #

GenBank: AAL05451.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000-1:100,000

ELISA

1:10,000-1:100,000

Western Blot

1:500

Protein

Uniprot #

Q9NZC7

Overview

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

Molecular Function

Coenzyme binding; protein dimerization activity; cofactor binding;

Subcellular Location

Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus

Expression

Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.

Structure

Interacts with TP53, p73/TP73 and MAPK8. Interacts with MAPT/TAU, RUNX2 and HYAL2 (By similarity). Forms a ternary complex with TP53 and MDM2. Interacts with ERBB4, LITAF and WBP1. Interacts with DVL1, DVL2 and DVL3. May interact with FAM189B and SCOTIN.

Alternative Nomenclature

FOR antibody

FOR II protein antibody

FOR1 antibody

FRA16D antibody

Fragile 16D oxido reductase antibody

Fragile site FRA16D oxidoreductase antibody

Putative oxidoreductase antibody

WOX1 antibody

WW domain containing oxidoreductase antibody

WW domain containing protein WWOX antibody

WW domain oxidoreductase antibody

WWOX antibody