General Information

Product

p63 Antibody

Description

Affinity Purified Tumor protein 63 isoform 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human p63 protein.

Accession #

NCBI:  NP_001108454

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H3D4

Overview

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes.

Molecular Function

Activator, Developmental protein, chromatin binding

Subcellular Location

Nucleus

Expression

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. 

Structure

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Alternative Nomenclature

AIS antibody

Amplified in squamous cell carcinoma antibody

B(p51A) antibody

B(p51B) antibody

Chronic ulcerative stomatitis protein antibody

CUSP antibody

DN p63 alpha 1 antibody

EEC3 antibody

Keratinocyte transcription factor antibody

KET antibody

LMS antibody

MGC115972 antibody

MGC192897 antibody

NBP antibody

OFC8 antibody

p40 antibody

p51 antibody

P51/P63 antibody

p53CP antibody

p63 antibody

P73 antibody

p73H antibody

p73L antibody

RHS antibody

SHFM4 antibody

TAp63alpha antibody

TP53CP antibody

TP53L antibody

TP63 antibody

TP73 antibody

Transformation related protein 63 antibody

Trp53rp1 antibody

Trp63 antibody

Tumor protein 63 antibody

Tumor protein p53-competing protein antibody

Tumor protein p63 deltaN isoform delta antibody

Tumor protein p73 antibody

General Information

Product

Protein Vestigial Antibody Affinity Purified

Description

Affinity Purified Vestigial Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 200-250 on D. melanogaster Vestigial protein.

Accession #

NCBI:  NP_523723.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q26366

Overview

Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.

Molecular Function

DNA binding; Protein C-terminus binding

Subcellular Location

Nucleus

Expression

Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.

Alternative Nomenclature

VG

VSTGL

VG21

DMEL/CG3830

CCG3830

General Information

Product

WWOX2 Antibody Affinity Purified

Description

Affinity Purified Abberant WW domain-containing Oxidoreductase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on WWOX2 protein.

Accession #

GenBank: AAL05451.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000-1:100,000

ELISA

1:10,000-1:100,000

Western Blot

1:500

Protein

Uniprot #

Q9NZC7

Overview

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

Molecular Function

Coenzyme binding; protein dimerization activity; cofactor binding;

Subcellular Location

Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus

Expression

Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.

Structure

Interacts with TP53, p73/TP73 and MAPK8. Interacts with MAPT/TAU, RUNX2 and HYAL2 (By similarity). Forms a ternary complex with TP53 and MDM2. Interacts with ERBB4, LITAF and WBP1. Interacts with DVL1, DVL2 and DVL3. May interact with FAM189B and SCOTIN.

Alternative Nomenclature

FOR antibody

FOR II protein antibody

FOR1 antibody

FRA16D antibody

Fragile 16D oxido reductase antibody

Fragile site FRA16D oxidoreductase antibody

Putative oxidoreductase antibody

WOX1 antibody

WW domain containing oxidoreductase antibody

WW domain containing protein WWOX antibody

WW domain oxidoreductase antibody

WWOX antibody

General Information

Product

KAP1 / TRIM28 Antibody Affinity Purified

Description

Affinity Purified Tripartite motif-containing 28 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 750-834 on mouse TRIM28 protein.

Accession #

GenBank: AAH89337

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5EBP9

Molecular Function

Chromo shadow domain binding; Krueppel-associated box domain binding; Protein kinase activity

Subcellular Location

Chromatin; Nuclear heterochromatin; Nucleus; Nuclear euchromatin; Nucleoplasm

Structure

Contains RING-type zinc finger

Alternative Nomenclature

E3 SUMO protein ligase TRIM28 antibody

KAP 1 antibody

KRAB associated protein 1 antibody

KRIP1 antibody

Nuclear corepressor KAP 1 antibody

RING finger protein 96 antibody

RNF96 antibody

TF1B antibody

TIF1 beta antibody

Transcription intermediary factor 1 beta antibody

TRIM28 antibody

Tripartite motif containing 28 antibody

General Information

Product

YES1 Antibody

Description

Affinity Purified tyrosine-protein kinase Yes Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Accession #

NCBI:  NP_005424

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07947

Overview

Regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation.

Molecular Function

ATP Binding, ion channel binding, non-membrane spanning protein tyrosine kinase activity, protein tyrosine kinase activity, enzyme binding

Subcellular Location

Cell membrane, cytoplasm, cytoskeleton. (Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway)

Expression

Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of the epidermis.

Alternative Nomenclature

c Yes antibody

Cellular Yes 1 protein antibody

HsT441 antibody

P61 YES antibody

Proto oncogene tyrosine protein kinase YES antibody

Proto-oncogene c-Yes antibody

Tyrosine-protein kinase Yes antibody

v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody

Viral oncogene yes 1 homolog 1 antibody

Yamaguchi sarcoma oncogene antibody

Yes antibody

YES1 antibody

General Information

Product

cIAP2 Antibody

Description

Affinity Purified baculoviral IAP repeat-containing protein 2 isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 545-620 on human cIAP2 protein.

Accession #

NCBI: NP_001157.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

Q134990

Molecular Function

Ligase activity; Transcription coactivator activity, Protein N-terminus binding

Subcellular Location

Cytoplasm; Nucleus

Expression

Is present in fetal and adult tissues and is mostly expressed in adult skeletal muscle, thymus, testis, ovary, and pancras.  Is low or absent in both brain and peripheral  blood leukocytes.

Structure

Interacts with DIABLO/SMAC. Interacts with CASP9.  Interacts with TRAF2; intercation required for IKBE ubiquination.

Alternative Nomenclature

AIP 1 antibody

API 2 antibody

Apoptosis inhibitor 2 antibody

Baculoviral IAP repeat containing 3 antibody

BIRC 3 antibody

HAIP 1 antibody

HIAP1 antibody

IAP homolog C antibody

MALT 2 antibody

Mammalian IAP homolog C antibody

MIHC antibody

RING finger protein 49 antibody

RNF49 antibody

TNFR2 TRAF signaling complex protein 1 antibody

General Information

Product

C9orf72 Antibody

Verified Applications

DB, ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on human C9orf72 isoform a protein.

Accession #

NCBI:  NP_001242983.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96LT7

Overview

May play a role in endosomal trafficking and autophagy

Molecular Function

Autophagy; endocytosis; cell death

Subcellular Location

Nucleus; Cytoplasm; Endosome; Lysosome; Autophagosome; Secreted

Expression

Both isoforms are widely expressed including kidney, lung, liver, heart, testis, cerebellum, frontal cortex and lymphoblasts.

Structure

Interacts with small Rab GTPases including RAB1A and RAB7A

Alternative Nomenclature

mALSFTD Antibody

chromosome 9 open reading frame 72 antibody

FTDals antibody

MGC23980 antibody

RP11-27J8.2 antibody

General Information

Product

Histone H3 Antibody

Description

Affinity Purified Histone H3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Pedicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human HH3 protein.

Accession #

GeneBank: CAA58540.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P68431

Overview

Core component of nucleosome.Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Molecular Function

DNA-binding

Subcellular Location

Nucleus; Chromosome

Expression

During Sphase, then expression decreases as cell division slows down during the process of differentiation

Structure

Belongs to histone H3 family

Alternative Nomenclature

H3.3B antibody

H3F3A antibody

H3 histone antibody

H3.3A antibody

H3F3 antibody

H3f3b antibody

HIST3H3 antibody

Histone H3 3 pseudogene antibody

PP781 antibody

General Information

Product

Histone H3 Antibody

Description

Affinity Purified Histone H3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, S. cerevisiae

Immunogen

Synthetic peptide taken within amino acid 1-50 on Sacchoromyces cerevisiae protein HH3 protein.

Accession #

Gen Bank: EDZ69636.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B5VR08

Overview

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro).

Molecular Function

Chromatin regulator, Methyltransferase, Transferase

Subcellular Location

Nucleus

Structure

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Contains 1 DOT1 domain.

Alternative Nomenclature

H3 histone family, member J antibody

H3 histone antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

H3 histone family, member C antibody

H3 histone family, member D antibody

H3 histone family, member F antibody

H3 histone family, member H antibody

H3 histone family, member I antibody

H3 histone family, member K antibody

H3 histone family, member L antibody

H3 histone, family 3A antibody

H3.3A antibody

H3F1K antibody

H3F3 antibody

Histone cluster 1, H3a antibody

Histone cluster 1, H3b antibody

Histone cluster 1, H3c antibody

Histone cluster 1, H3d antibody

Histone cluster 1, H3e antibody

Histone cluster 1, H3f antibody

Histone cluster 1, H3g antibody

Histone cluster 1, H3i antibody

Histone cluster 1, H3j antibody

General Information

Product

IRE1A Antibody

Description

Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody

Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
inositol-requiring enzyme 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody

MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody