General Information

Product

GCN2 Antibody

Description

Affinity Purified eIF2alpha kinase Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1700 on human GCN2 protein.

Accession #

GenBank:  CAB58363.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9P2K8

Overview

Can phosphorylate alpha subunit of EIF2 and mediates translational control.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Cytosolic ribosome

Expression

Liver, brain.

Structure

Contains 2 protein kinase domains; Contains 1 RWD domain.

Alternative Nomenclature

Eif2ak4 antibody

Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody

GCN2 antibody

GCN2 eIF2alpha kinase antibody

GCN2 like protein antibody

KIAA1338 antibody

MGCN2 antibody

General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody

General Information

Product

LIM kinase 2 Antibody

Description

Affinity Purified Lysine-specific demethylase 4C Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 570-638 on human LIMK2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53671

Overview

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code

Molecular Function

Androgen receptor binding, enzyme binding, histone demethylase activity, zinc ion binding, dioxygenase activity

Subcellular Location

Nucleus

Expression

Overexpressed in several esophageal squamous cell carcinomas (ESCs).

Alternative Nomenclature

LIM domain kinase 2 antibody

LIMK 2 antibody

General Information

Product

SPHK1 Antibody

Description

Affinity Purified Sphingosine kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.

Accession #

GenBank: CAM17117.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8CI15

Overview

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Molecular Function

ATP binding, calmodulin binding, DNA binding, magnesium ion binding, NAD+kinase activity, sphinganine kinase activity

Subcellular Location

Cytoplasm, nucleus, cell membrane

Alternative Nomenclature

SK1 antibody

Sphingosine Kinase 1 antibody

SPHK 1 antibody

SPHK antibody

SPK antibody

SPK 1 antibody

General Information

Product

AKT1 Antibody

Description

Affinity Purified RAC-alpha serine/threonine-protein kinase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within the last 50 amino acid on human RAC-alpha serine/threonine-protein kinase protein.

Accession #

GeneBank: AAA36539

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

PKB antibody

Protein Kinase B Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

General Information

Product

BCKDH Antibody

Description

Affinity Purified Branched chain a-ketoacid dehydrogenase kinase Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 170-200 on human BCKD protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O14874

Overview

Catalyzes phosphorylation and inactivation of branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine.

Molecular Function

Kinase; Transferase; ATP-binding; Nucleotide-binding

Subcellular Location

Mitochondrion Matrix

Expression

Ubiquitious

Structure

Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Alternative Nomenclature

[3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody

mitochondrial antibody

General Information

Product

ILK Antibody

Description

Affinity Purified Integrin-linked protein kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-452 on human Integrin-linked protein kinase.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.87 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13418

Overview

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.

Molecular Function

ATP binding, protein kinase binding, protein serine/threonine kinase activity, signal transducer activity

Subcellular Location

Cell junction, cell membrane, peripheral membrane protein, cytoplasmic side, cell projection

Expression

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Alternative Nomenclature

59 kDa serine/threonine protein kinase antibody

DKFZp686F1765 antibody

ILK 1 antibody

ILK 2 antibody

ILK antibody

Integrin linked kinase 2 antibody

Integrin-linked protein kinase antibody

p59 antibody

p59ILK antibody

General Information

Product

ITK Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-600 on human ITK protein.

Accession #

GenBank: BAA02873.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q08881

Overview

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.

Molecular Function

ATP binding, metal ion binding, non-membrane spanning protein tyrosine kinase activity

Subcellular Location

Cytoplasm (Note: Localizes in vicinity of cell surface receptors in plasma membrane after receptor stimulation)

Expression

T-cell lines and natural killer cell lines.

Alternative Nomenclature

EMT antibody

Homolog of mouse T cell itk/tsk antibody

IL 2 inducible T cell kinase antibody

Itk antibody

Kinase EMT antibody

LPFS1 antibody

LYK antibody

MGC126257 antibody

MGC126258 antibody

PSCTK 2 antibody

T cell specific kinase antibody

TSK antibody

Tyrosine protein kinase antibody

Tyrosine protein kinase ITK/TSK antibody

General Information

Product

MAP3K8 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 8 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-467 on human MAP3K8 protein.

Accession #

GenBank: AAI13567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P41279

Overview

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Expressed in several normal tissues and human tumor-derived cell lines.

Structure

Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KRS2; the interaction inhibits ERK and NF-kappa-B activation.

Alternative Nomenclature

c COT antibody

Cancer Osaka thyroid oncogene antibody

COT antibody

COT proto oncogene serine/threonine protein kinase antibody

EST antibody

ESTF antibody

Ewing sarcoma transformant antibody

FLJ10486 antibody

MEKK8 antibody

Mitogen activated protein kinase kinase kinase 8 antibody

Proto oncogene cCot antibody

Serine/threonine-protein kinase cot antibody

TPL 2 antibody

Tumor progression locus 2 antibody

General Information

Product

mTOR Antibody Affinity Purified

Description

Affinity Purified Serine/threonine-protein kinase mTOR Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-480 on mTOR protein.

Accession #

NCBI:  NP_063971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42346

Overview

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Alternative Nomenclature

FK506 binding protein 12 rapamycin associated protein 1 antibody

FKBP rapamycin associated protein antibody

FRAP1 antibody

FRAP2 antibody

mTOR antibody

RAFT1 antibody

Rapamycin target protein antibody

RAPT1 antibody

Serine/threonine-protein kinase mTOR antibody