General Information

Product

ROCK2 Antibody

Description

Affinity Purified Rho-associated protein kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1300-1388 on human ROCK2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O75116

Overview

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Nucleus

Structure

Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.

Alternative Nomenclature

coiled-coil-containing protein kinase 2 antibody

KIAA0619 antibody

p164 ROCK 2 antibody

Rho associated, coiled coil containing protein kinase II antibody

ROK alpha antibody

General Information

Product

EDG8 Antibody

Description

Affinity Purified Sphingosine 1-phosphate receptor 5 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 380-400 of rat S1P5R/EDG8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9JKM5

Overview

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of

Alternative Nomenclature

EDG 8 antibody

Endothelial differentiation G protein coupled receptor 8 antibody

Endothelial differentiation sphingolipid G protein coupled receptor 8 antibody

S1P receptor 5 antibody

S1P receptor Edg 8 antibody

Sphingosine 1 Phosphate Receptor 5 antibody

Sphingosine 1-phosphate receptor Edg-8 antibody

SPPR2 antibody

General Information

Product

Solute carrier family 35 member E4 Antibody Affinity Purified

Description

Affinity Purified Solute carrier family 35, member E4 (Slc35e4), mRNA Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 170-200 on Slc35E4 protein.

Accession #

NCBI: NM_153316.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5RKL7

Overview

Putative transporter

Subcellular Location

Membrane

Structure

Belongs to the TPT transporter family. SLC35E subfamily

Alternative Nomenclature

MGC129826 antibody

SLC35E4 antibody

General Information

Product

Sodium / Hydrogen Exchanger 3 Antibody Affinity Purified

Description

Affinity Purified Sodium/Hydrogen Exchanger 3 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 790-835 on Slc9A3 protein.

Accession #

NCBI:  NP_004165.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P48764

Overview

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Molecular Function

PDZ domain binding; Sodium:proton antiporter activity

Subcellular Location

Apical cell membrane; Multi-pass membrane protein

Structure

Binds SLC9A3R1 and SLC9A3R2. Interacts with CHP1, CHP2 and SHANK2. Interacts with PDZK1 (via C-terminal PDZ domain) (By similarity). Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels.

Alternative Nomenclature

MGC126718 antibody

MGC126720 antibody

NHE3 antibody

SLC9A 3 antibody

Sodium/hydrogen exchanger 3 antibody

Solute carrier family 9 member 3 antibody

General Information

Product

SPHK1 Antibody

Description

Affinity Purified Sphingosine kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.

Accession #

GenBank: CAM17117.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8CI15

Overview

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Molecular Function

ATP binding, calmodulin binding, DNA binding, magnesium ion binding, NAD+kinase activity, sphinganine kinase activity

Subcellular Location

Cytoplasm, nucleus, cell membrane

Alternative Nomenclature

SK1 antibody

Sphingosine Kinase 1 antibody

SPHK 1 antibody

SPHK antibody

SPK antibody

SPK 1 antibody

General Information

Product

Topoisomerase II alpha Antibody

Description

Affinity Purified DNA topoisomerase (ATP-hydrolysing) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

Accession #

GenBank: CAA09762.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11388

Overview

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Molecular Function

Isomerase, Topoisomerase, ATP binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity.

Alternative Nomenclature

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

TOP2 antibody

TOP2A antibody

Topoisomerase DNA II alpha 170kDa antibody

TP2A antibody

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

General Information

Product

UCN2 Antibody Affinity Purified

Description

Affinity Purified Urocortin-2 preprotein Antibody

Verified Applications

ELISA, SB, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken wtihin amino acid region 70-115 of human Urocortin 2 protein.

Accession #

NCBI:  NP_149976.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.71 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96RP3

Overview

Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.

Molecular Function

Hormone binding, receptor binding, hormone

Subcellular Location

Secreted

Expression

Binds with high affinity to CRF receptors 2-alpha and 2-beta.

Alternative Nomenclature

SRP

Stresscopin-related peptide

Ucn II

UCN2

UCNI

UR

Urocortin II

Urocortin-2

Urocortin-related peptide

URP

General Information

Product

Aquaporin 12 Antibody

Verified Applications

ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 240-290 on mouse Aquaporin 12 protein.

Accession #

GenBank:  EDL39975

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61-0.67 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q3V2I1

Overview

Water transport activity

Molecular Function

Transport

Subcellular Location

Membrane

Expression

Cytoplasm; plasma membrane; integral component of membrane

Structure

Contain two tandem repeats each with three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala

Alternative Nomenclature

AQP 12 antibody

AQP 12A antibody

AQPX2 antibody

Aquaporin 12 antibody

Aquaporin 12A antibody

Aquaporin X2 antibody

General Information

Product

PDE7A Antibody

Description

Affinity Purified cAMP-specific cyclic nucleotide phosphodiesterase PDE7A3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE7A variants

Accession #

GenBank: AAK57640.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

Q13946

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.

Molecular Function

3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Isoform PDE7A1 : Cytoplasm › cytosol, Isoform PDE7A2: Cytoplasm

Expression

PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.

Structure

Interacts with CBFA2T3

Alternative Nomenclature

HCP 1 antibody

P2A antibody

PDE 7 antibody

PDE1/PDE2 yeast human complement of antibody

PDE7A antibody

Phosphodiesterase 7A antibody

Phosphodiesterase isozyme 7 antibody

Rolipram insensitive phosphodiesterase type 7 antibody

TM 22 antibody

General Information

Product

Phospho-PDE7A Antibody

Description

Affinity Purified High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated Synthetic cyclic peptide common to all PDE7A variants

Specificity

This antibody is specific for PDE7A variants and will not cross-react with PDE7B or other PDE family members.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P70453

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.

Molecular Function

Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Cytoplasm

Expression

PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.

Structure

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Alternative Nomenclature

HCP 1 antibody

P2A antibody

PDE 7 antibody

PDE 7A antibody

PDE1/PDE2 yeast human complement of antibody

PDE7 antibody

PDE71 antibody

Phosphodiesterase 7A antibody

Phosphodiesterase isozyme 7 antibody

Rolipram insensitive phosphodiesterase type 7 antibody

TM22 antibody