General Information

Product

Orexin A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on ORXN protein.

Accession #

NCBI: NP_034540

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O55241

Overview

Is a portion of a protein complex (ribonuclease P) that creates mature tRNA molecues through the cleavage of their 5' ends.

Molecular Function

ribonuclease P activity, Endonuclease, Hydrolase, Nuclease

Subcellular Location

Cytoplasm

Structure

Contains a catalytic RNA component and at least 4 to 5 protein subunits.

Alternative Nomenclature

HCRT 1 antibody

HCRT antibody

HCRT2 antibody

Hypocretin 1 antibody

Hypocretin 2 antibody

Hypocretin antibody

Hypocretin neuropeptide precursor antibody

NRCLP1 antibody

Orexin A antibody

Orexin antibody

Orexin B antibody

OX antibody

PPORX antibody

PPOX antibody

PREPROOREXIN antibody

General Information

Product

SESN1 Antibody

Description

Affinity Purified Sestrin-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human SESN1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y6P5

Overview

Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth.

Molecular Function

Cell cycle arrest; Cellular response to DNA damage stimulus; Gene expression; Regulation of protein kinase B signaling; Transcription initation from RNA polymerase II promoter; Regulation of response to reactive oxygen species; Negative regulation of cell.

Subcellular Location

Nucleus

Expression

Widely Expressed

Structure

Belongs to the Sestrin family.

Alternative Nomenclature

1110002G11Rik antibody

AU044290 antibody

p53 activated gene 26 antibody

p53 regulated PA26 nuclear protein antibody

PA26 antibody

RP11-787I22.1 antibody

sesn1 antibody

SEST1 antibody

Sestrin-1 antibody

General Information

Product

SESN2 Antibody

Description

Affinity Purified Sestrin 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-80 on SESN2 protein.

Accession #

GenBank: AAM92261.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P58004

Overview

Involved in the reduction of peroxiredoxins.

Subcellular Location

Cytoplasm, Nucleus

Expression

Widely expressed.

Alternative Nomenclature

Hi95 antibody

Hypoxia induced gene 95 antibody

RP23 63D8.3 antibody

SES2 antibody

SEST2 antibody

General Information

Product

SIRT1 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid 25-75 on rat Sirtuin 1 protein.

Accession #

NCBI:  NP_062786.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.69-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q923E4

Overview

NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autphagy.

Subcellular Location

Nucleus, PML body, cytoplasm. (Nucleus: Recruited to the nuclear bodies via its interaction with the PML. Colocalized with APEX1 in nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm).

Expression

Widely expressed. Weakly expressed in liver and skeletal muscle.

Alternative Nomenclature

75SirT1 antibody

HST2, S. cerevisiae, homolog of antibody

NAD dependent deacetylase sirtuin 1 antibody

Regulatory protein SIR2 homolog 1 antibody

SIR2 like protein 1 antibody

SIR2, S.cerevisiae, homolog-like 1 antibody

SIR2ALPHA antibody

SIR2L1 antibody

SirtT1 75 kDa fragment antibody

Sirtuin type 1 antibody

General Information

Product

SREBP1 Antibody Affinity Purified

Description

Affinity Purified Sterol regulatory element-binding protein 1 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 950-1000 on human Sterol regulatory element binding transcription factor 1 protein.

Accession #

GenBank: AAH63281.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P36956

Overview

Transcriptional activator required for lipid homeostasis.

Molecular Function

Activator

Subcellular Location

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Expression

liver, adrenal gland, lung, ovary, hepatoma cell lines, kidney, brain, white fat, and muscle.

Structure

Belongs to the SREBP family

Alternative Nomenclature

ADD 1 antibody

Class D basic helix-loop-helix protein 1 antibody

Processed sterol regulatory element-binding protein 1 antibody

SREBF 1 antibody

SREBP1 antibody

Sterol regulatory element-binding transcription factor 1 antibody

General Information

Product

SREBP2 Antibody

Description

Affinity Purified Sterol regulatory element binding protein-2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.

Accession #

GenBank: AAA50746.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q12772

Overview

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

Molecular Function

Activator, E-box binding, protein C-terminus binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein, Golgi apparatus membrane; Multi-pass membrane protein, Cytoplasmic vesicle > COPII-coated vesicle membrane; Multi-pass membrane protein

Expression

Ubiquitously expressed in adult and fetal tissues.

Structure

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF13.

Alternative Nomenclature

AI608257 antibody

bHLHd2 antibody

Class D basic helix-loop-helix protein 2 antibody

Processed sterol regulatory element-binding protein 2 antibody

SREBF 2 antibody

SREBP2 antibody

Sterol regulatory element-binding transcription factor 2 antibody

General Information

Product

RIPK2 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 2 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey

Immunogen

Synthetic peptide taken within amino acid region 410-460 on human RIPK2 protein.

Accession #

GenBank: AAQ89173.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O43353

Overview

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Molecular Function

ATP binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; Protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity

Subcellular Location

Cytoplasm

Expression

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Structure

Found in a signaling complex consisting of at least ARHGEF2, NOD2 and RIPK2. Interacts with ARHGEF2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Binds to CFLAR/CLARP and CASP1 via their CARD domains. Binds to BIRC3/c-IAP1 and BIRC2/c-IAP2, TRAF1, TRAF2, TRAF5 and TRAF6. May be a component of both the TNFRSF1A and TNRFSF5/CD40 receptor complex. Interacts with NOD1. Interacts (via CARD domain) with NOD2 (via CARD domain). Interacts with MAP3K4; this interaction sequesters RIPK2 from the NOD2 signaling pathway. Interacts with IKBKG/NEMO. The polyubiquitinated protein interacts with MAP3K7/TAK1. Interacts with XIAP/BIRC4. Interacts with NLRP10. Interacts with CARD9.

Alternative Nomenclature

CARD carrying kinase antibody

CARD CONTAINING ICE ASSOCIATED KINASE antibody

CARD containing interleukin 1 beta converting enzyme associated kinase antibody

CARDIAK antibody

CLARP kinase antibody

GIG 30 antibody

Growth inhibiting gene 30 antibody

Receptor interacting protein 2 antibody

Receptor interacting serine threonine kinase 2

RICK antibody

RIP-like-interacting CLARP kinase antibody

General Information

Product

FNDC5 Antibody

Description

Affinity Purified Fibronectin type III domain-containing protein 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on human FNDC5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8K4Z2

Overview

Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.

Molecular Function

Hormone

Subcellular Location

Cell membrane, Membrane, Peroxisome, Secreted

Expression

highly expressed in skeletal muscle, heart and brain.

Alternative Nomenclature

Fibronectin type III domain containing 5 antibody

FNDC 5 antibody

FRCP2 antibody

irisin antibody

General Information

Product

SOCS3 Antibody

Description

Affinity Purified Suppresor of Cytokine Signaling 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 175-225 on human SOCS3 protein.

Accession #

GenBank:  CAG46495

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q6FI39

Molecular Function

Protein modification; protein ubiquitination.

Subcellular Location

Cytoplasm

Expression

Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Alternative Nomenclature

ATOD4 antibody

CIS 3 antibody

Cish3 antibody

Cytokine induced SH2 protein 3 antibody

E2a Pbx1 target gene in fibroblasts 10 antibody

EF 10 antibody

SOCS 3 antibody

SSI3 antibody

STAT induced STAT inhibitor 3 antibody

Suppressor of cytokine signaling 3 antibody

General Information

Product

PERK Antibody

Description

Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.

Accession #

NCBI:  NP_004827

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9NZJ5

Overview

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Expression

Ubiquitous. A high level expression is seen in secretory tissues.

Structure

Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.

Alternative Nomenclature

DKFZp781H1925 antibody

E2AK3_HUMAN antibody

EC 2.7.11.1 antibody

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

PEK antibody

PRKR like endoplasmic reticulum kinase antibody

WRS antibody