General Information

Product

Bestrophin 2 Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 388-404 of Mouse Bestrophin 2 protein, peptide was covalently modified.

Sequence:

EVHGDFLQRLLPAGAGS

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8BGM5

Overview

Forms calcium sensitive chloride channels. Highly permeable to bicarbonate.

Molecular Function

Ion transport; Sensory transduction; Transport

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Retinal pigment epithelium and colon

Structure

Belongs to Bestrophin Family

Alternative Nomenclature

BEST 2 antibody

Vitelliform macular dystrophy 2 homolog antibody

Vitelliform macular dystrophy 2 like 1 antibody

VMD2L1 antibody

General Information

Product

Phospho inaD Antibody Affinity Purified

Description

Affinity Purified Phospho Inactivation no afterpotential D, isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 600-700 on iNAD protein.

Accession #

NCBI:  NP_726260.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500

Protein

Uniprot #

Q24008

Overview

Utilizes a calcium-mediated process to function in the negative feedback regulation of the light activated signalling cascade in photoreceptors.

Molecular Function

Calmodulin binding, myosin binding, photoreceptor activity

Subcellular Location

Cell membrane, Peripheral membrane protein

Expression

Expression is detected in rhabdomers of the compound eyes and ocelli

Structure

Interacts with the C-terminus of trp.  Forms the inaD signalling complex through its interaction  with norpA and inaC.

Alternative Nomenclature

Inactivation-no-after-potential D protein antibody

inaD antibody

INAD_DROME antibody

General Information

Product

Capricious Antibody Affinity Purified

Description

Affinity Purified Capricious, isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to 400-450 on Capricious isoform A protein.

Accession #

NCBI:AAF49837

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9VU53

Overview

Invovled in axon guidance, branch fusion, opening tracheal system, synapse assembly and others.

Subcellular Location

Muscle cell projection

Structure

Contains Leucine-rich repeat

Alternative Nomenclature

BcDNA:LD07388 antibody

caps antibody

CG11282 antibody

CT31495 antibody

Dmel\CG11282 antibody

l(3)02937 antibody

l(3)rM632 antibody

General Information

Product

trp Antibody Affinity Purified

Description

Affinity Purified Transient Receptor Potential isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Transient Receptor Potential isoform A protein.

Accession #

NCBI:  NP_476768.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:10,000

Protein

Uniprot #

P19334

Overview

A light-sensitive calcium channel that is required for inositide-mediated Ca2+ entry in the retina during phospholipase C (PLC)-mediated phototransduction. Ca2+ influx may then feed back and inhibit PLC, thereby facilitating phosphatidylinositol 4,5 bisphosphate (PIP2) recycling.

Molecular Function

Calcium channel, Ion channel, Calmodulin activity

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Expressed predominantly in the rhabdomeres of photoreceptor cells. Expressed in the third antennal segment and in the olfactory segment at approximately 70 hours after puparium formation during antennal development.

Structure

The C-terminus interacts with a PDZ domain of inaD to form the core of the inaD signaling complex. Other members of the complex include norpA (PLC), inaC (PKC), and possibly trpl, ninaC, FKBP59, calmodulin and rhodopsin. Forms homomultimers and heteromult

Alternative Nomenclature

DmTRP antibody

dTRP antibody

Transient receptor potential antibody

General Information

Product

SFRP5 Antibody

Description

Affinity Purified Secreted frizzled-related protein 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on SFRP5

Accession #

GenBank: AAD25052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5T4F7

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Molecular Function

PDZ domain binding, Wnt-protein binding, Wnt-activated receptor activity

Subcellular Location

Secreted

Expression

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle.

Alternative Nomenclature

Frizzled related protein 1b

FRP 1b

SARP3

Secreted apoptosis related protein 3

Secreted frizzled related protein 5

General Information

Product

Phospho trp Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Transient Receptor Potential isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Transient Receptor Potential isoform A protein.

Accession #

NCBI:  NP_476768.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:10,000

Protein

Uniprot #

P19334

Overview

A light-sensitive calcium channel that is required for inositide-mediated Ca2+ entry in the retina during phospholipase C (PLC)-mediated phototransduction. Ca2+ influx may then feed back and inhibit PLC, thereby facilitating phosphatidylinositol 4,5 bisphosphate (PIP2) recycling.

Molecular Function

Calcium channel, Ion channel, Calmodulin activity

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Expressed predominantly in the rhabdomeres of photoreceptor cells. Expressed in the third antennal segment and in the olfactory segment at approximately 70 hours after puparium formation during antennal development.

Structure

The C-terminus interacts with a PDZ domain of inaD to form the core of the inaD signaling complex. Other members of the complex include norpA (PLC), inaC (PKC), and possibly trpl, ninaC, FKBP59, calmodulin and rhodopsin. Forms homomultimers and heteromult

Alternative Nomenclature

DmTRP antibody

dTRP antibody

Transient receptor potential antibody

General Information

Product

RPE65 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on mouse RPE65 protein.

Accession #

GenBank: AAL01119.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q91ZQ5

Overview

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester.

Molecular Function

Hydrolase, Isomerase

Subcellular Location

Cytoplasm, Cell membrane; Lipid anchor, Microsome membrane

Expression

Retinal pigment epithelium  specific

Structure

Interacts with MYO7A; this mediates light-dependent intracellular transport of RPE65.

Alternative Nomenclature

All-trans-retinyl-palmitate hydrolase antibody

LCA 2 antibody

Leber congenital amaurosis antibody

p63 antibody

rd 12 antibody

Retinal pigment epithelium specific 61 kDa protein antibody

Retinal pigment epithelium specific 65 kDa protein antibody

retinitis pigmentosa 20 antibody

Retinoid isomerohydrolase antibody

RP20 antibody

General Information

Product

USH2A Antibody

Description

Affinity Purified Usherin isoform A Antibody Mid-Region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 255-305 on human Usherin isoform A protein.

Accession #

NCBI:  NP_009054.5

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500. Detects apparent band of 186 kDa.

Protein

Uniprot #

O75445

Overview

Involved in hearing and vision.

Molecular Function

collagen binding, myosin binding

Subcellular Location

Cell projection › stereocilium membrane; Single-pass type I membrane protein

Expression

Present in the basement membrane of many, but not all tissues. Expressed in retina, cochlea, small and large intestine, pancreas, bladder, prostate, esophagus, trachea, thymus, salivary glands, placenta, ovary, fallopian tube, uterus and testis.

Structure

Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with USH1C and WHRN. Interacts with NINL. Interacts with PDZD7.

Alternative Nomenclature

dJ1111A8.1 antibody

US1 antibody

USH1A antibody

Usher syndrome type Ia protein antibody

Usher syndrome type-1A protein antibody

Usherin antibody

General Information

Product

Usherin Antibody

Description

Affinity Purified Usherin syndrome 2A Antibody N-epitope

Verified Applications

ELISA, IP

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-180 on Usherin syndrome 2A protein.

Accession #

NCBI:  NP_009054.5

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75445

Overview

Involved in hearing and vision.

Molecular Function

collagen binding, myosin binding

Subcellular Location

Cell projection › stereocilium membrane; Single-pass type I membrane protein

Expression

Present in the basement membrane of many, but not all tissues. Expressed in retina, cochlea, small and large intestine, pancreas, bladder, prostate, esophagus, trachea, thymus, salivary glands, placenta, ovary, fallopian tube, uterus and testis.

Structure

Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with USH1C and WHRN. Interacts with NINL. Interacts with PDZD7.

Alternative Nomenclature

dJ1111A8.1 antibody

US1 antibody

USH1A antibody

Usher syndrome type Ia protein antibody

Usher syndrome type-1A protein antibody

Usherin antibody

General Information

Product

Usherin Antibody

Description

Affinity Purified Usherin isoform A Antibody C-epitope

Verified Applications

ELISA, ICC/IF, IP

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1495-1545 on human Usherin isoform A protein.

Accession #

NCBI:  NP_009054.5

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75445

Overview

Involved in hearing and vision.

Molecular Function

collagen binding, myosin binding

Subcellular Location

Cell projection › stereocilium membrane; Single-pass type I membrane protein

Expression

Present in the basement membrane of many, but not all tissues. Expressed in retina, cochlea, small and large intestine, pancreas, bladder, prostate, esophagus, trachea, thymus, salivary glands, placenta, ovary, fallopian tube, uterus and testis.

Structure

Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with USH1C and WHRN. Interacts with NINL. Interacts with PDZD7.

Alternative Nomenclature

dJ1111A8.1 antibody

US1 antibody

USH1A antibody

Usher syndrome type Ia protein antibody

Usher syndrome type-1A protein antibody

Usherin antibody