General Information

Product

PINK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase PINK1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human PINK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BXM7

Overview

During cellular stress it serves to protect against mitochondrial dysfunction through the phosphorylation of mitochondrial proteins. Uses selective autophagy to clear mitochondria that has been damaged.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein, Cytoplasm

Expression

Expression is detected at high levels in the heart, skeletal muscles and testis.  Expression is detected at lower levels in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.

Structure

Interacts with PARK2 and FBXO7

Alternative Nomenclature

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

Protein kinase BRPK antibody

PTEN induced putative kinase 1 antibody

Serine/threonine-protein kinase PINK1 antibody

General Information

Product

PKC epsilon Antibody

Description

Affinity Purified Protein Kinase C epsilon Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Protein Kinase C epsilon type protein.

Accession #

NCBI:  NP_005391.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02156

Overview

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardio myocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAP, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implication the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaciton with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a rolei n the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). invovled in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be invovled in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin-and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. IS able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines produciton. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Cell Membrane; Nucleus

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative Nomenclature

KPCE_HUMAN antibody

MGC125656 antibody

MGC125657 antibody

nPKC epsilon antibody

nPKC-epsilon antibody

PKCE antibody

Pkcea antibody

PRKCE antibody

Protein kinase C epsilon antibody

General Information

Product

cGKI Antibody

Description

Affinity Purified cGMP-dependent protein kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 609-659 on human protein kinase G alpha subunit.

Accession #

NCBI:  NP_001091982.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13976

Overview

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Primarily expressed in lung and placenta

Structure

Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A.

Alternative Nomenclature

cGK 1 antibody

cGKI alpha antibody

CGKI antibody

cGKI beta antibody

cGMP-dependent protein kinase 1 antibody

DKFZp686K042 antibody

FLJ36117 antibody

KGPB antibody

MGC71944 antibody

pkg antibody

PRKG1 antibody

PRKGR1A antibody

PRKGR1B antibody

General Information

Product

Phospho-PDE4D PKA site (S190) Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 4D PKA site Antibody

Verified Applications

ELISA, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide selective for PDE4D PKA site taken within amino acid region 50-100 on human PDE4D8 protein. Phosphorylated Serine 190 residue. Derived around the PKA phosphorylation site of serine 190:

R-E-S^P-F-L

Accession #

NCBI:  NP_001184148.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q08499

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

5''-cyclic phosphodiesterase 4D antibody

cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody

cAMP specific phosphodiesterase 4D antibody

cAMP specific phosphodiesterase PDE4D antibody

cAMP specific phosphodiesterase PDE4D6 antibody

cAMP-specific 3'' antibody

cyclic AMP phosphodiesterase antibody

DPDE3 antibody

Dunce like phosphodiesterase E3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE43 antibody

PDE4DN2 antibody

Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody

Phosphodiesterase 4D cAMP specific antibody

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK 1 antibody

General Information

Product

PREX2 Antibody Affinity Purified

Description

Affinity Purified PtdIns(3,4,5)-dependent Rac exchanger 2a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 720-900 on PREX2 protein.

Accession #

GenBank:  DAA05334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q70Z35

Overview

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.

Molecular Function

Rac GTPase activator activity, Rac guanyl-nucleotide exchange factor activity

Expression

Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.

Alternative Nomenclature

4 antibody

5)-dependent Rac exchanger 2 antibody

5-trisphosphate-dependent Rac exchanger 2 protein antibody

DEP domain-containing protein 2 antibody

DEP.2 antibody

Phosphatidylinositol 3 antibody

PtdIns(3,4,5)-dependent Rac exchanger 2 antibody

General Information

Product

Phospho-TICAM2 Antibody

Description

Affinity Purified Phosphorylated TRIF-related adapter molecule Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule protein.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and dirupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in time-dependent manner. Interacts with IL18R1 and IL8RAP.

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TIR containing adapter molecule 2 antibody

TIR domain containing adaptor protein antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody

General Information

Product

RIPK4 Antibody

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein 

kinase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human RIPK4 protein.

Accession #

NCBI:  NP_065690.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9H4D1

Overview

ATP binding; Protein Serine/Threonine kinase activity

Structure

Contains 10 ANK repeats

Alternative Nomenclature

ANKK2 antibody

ANKRD 3 antibody

Ankyrin repeat domain 3 antibody

DIK antibody

PKC delta interacting protein kinase antibody

PKK antibody

protein kinase C-associated kinase antibody

Receptor interacting serine/threonine protein kinase 4 antibody

Serine/threonine protein kinase ANKRD3 antibody

General Information

Product

ROR1 Antibody

Description

Affinity Purified Receptor tyrosine kinase-like orphan receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to C terminal amino acids 900-918 on Human ROR1 protein. Peptide was coupled to a carrier protein using heterobifunctional cross linker.

Sequence:

TVKGNKSQKPYKIDSKQAS

Accession #

GenBank:  EAX06557.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0000EE3178

Overview

Tyrosine-protein kinase receptor whose role is not yet clear.

Molecular Function

ATP binding; transmembrane receptor protein Tyrosine kinase activity; Wnt-protein binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.

Structure

Contains 1 FZ (frizzled) domain; Contains 1 Ig-like C2-type (immunoglobulin-like) domain; Contains 1 Kringle domain; Contains 1 Protein kinase domain

Alternative Nomenclature

dJ537F10.1 antibody

MGC99659 antibody

Neurotrophic tyrosine kinase antibody

Receptor tyrosine kinase like orphan receptor 1 antibody

receptor-related 1 antibody

RGD1559469 antibody

RP11 24J23.1 antibody

Tyrosine kinase like orphan receptor 1 antibody

General Information

Product

ROR2 Antibody

Description

Affinity Purified Tyrosine-protein kinase transmembrane receptor ROR2 precursor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-943 on human ROR2 protein.

Accession #

NCBI:  NP_004551.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q01974

Overview

Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation.

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass type 1 membrane  protein

Expression

Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.

Structure

Homodimer; promotes osteogenesis. Binds YWHAB. Interacts with WTIP.

Alternative Nomenclature

BDB antibody

BDB1 antibody

Brachydactyly type B antibody

EC 2.7.10.1 antibody

MGC163394 antibody

Neurotrophic tyrosine kinase, receptor related 2 antibody

NTRKR2 antibody

Receptor tyrosine kinase-like orphan receptor 2 antibody

General Information

Product

ULK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK1 Antibody

Verified Applications

ELISA, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Panda

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on mouse ULK1 protein.

Accession #

NCBI:  NP_003556.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

O75385

Overview

Involved in autophagy in response to starvation. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Molecular Function

ATP Binding, protein serine/threonine kinase activity, Rab GTPase binding, protein complex binding

Subcellular Location

Cytoplasm, cytosol, preautphagosomal structure. (Note: under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome)

Expression

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Alternative Nomenclature

ATG1 autophagy related 1 homolog antibody

ATG1A antibody

Autophagy related protein 1 homolog antibody

hATG1 antibody

Serine/threonine-protein kinase ULK1 antibody

ULK1 antibody

Unc 51 like kinase 1 antibody

Unc-51 like kinase 1 (C. elegans) antibody

UNC51 antibody