General Information

Product

ULK2 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-600 on rat ULK2 protein.

Accession #

NCBI:  NP_001178574.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

D3ZUD3

Overview

Regulation of actin dynamics for phagocytic cup formation.

Alternative Nomenclature

ATG1B antibody

KIAA0623 antibody

Serine/threonine protein kinase ULK2 antibody

ULK2 antibody

Unc 51 like autophagy activating kinase 2 antibody

Unc 51 like kinase 2 antibody

General Information

Product

ULK3 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK3 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to a region within C terminal amino acids 452-472 of Mouse ULK3, coupled to a carrier protein.

Accession #

NCBI:  NP_001258064.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

D3ZHP7

Overview

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Molecular Function

ATP-binding; Nucleotide-binding; Serine/threonine-protein kinase

Subcellular Location

Cytoplasm

Expression

Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.

Structure

Interacts (via protein kinase domain) with SUFU.

Alternative Nomenclature

DKFZP434C131 antibody

FLJ90566 antibody

Serine/threonine-protein kinase ULK3 antibody

Ulk3 antibody

unc 51 like kinase 3 (C. elegans) antibody

Unc-51-like kinase 3 antibody

General Information

Product

Phospho Lyn Antibody Affinity Purified

Description

Affinity Purified Tyrosine-protein kinase Lyn isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 425-525 on human Lyn protein. Phospho Y 491.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

LYN proto oncogene, Src family tyrosine kinase antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

Phospho-BTK Antibody (Y551)

Description

Affinity Purified Phospho-specific Bruton's Tyrosine Kinase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide corresponding to Human BTK amino acids 544-561 (phopsho Y551). 

Sequence 

RYV LDD EY(p)T SSV GSK FPV

Accession #

NCBI:  NP_000052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500. Predicted molecular weight: 76 kDa.

Protein

Uniprot #

Q06187

Overview

Non-receptor tyrosine kinase invovled in B lymphocyte development.

Molecular Function

Kinase; Transferase; Tyrosine-protein Kinase

Subcellular Location

Cytoplasm; Cell Membrane; Peripheral membrane: Nucleus.  In steady state BTK is predominantly cytosolic.

Expression

B-lymphocytes

Structure

PH domain mediates binding to inositol polyphosphate and phosphoinositides, leading to its targeting with plasma membrane.  Extended in the BTK kinase family by a region designated Tec homology domain, which has roughly 80 residues preceding SH3 domain.

Alternative Nomenclature

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

MGC126261 antibody

MGC126262 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

General Information

Product

CAB39 Antibody

Description

Calcium Binding Protein 39 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human CAB39 protein.

Accession #

NCBI:  BC010652.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y376

Overview

Component of a complex that binds and activates STK11/LKB1.

Molecular Function

Kinase binding, protein kinase activator activity, protein serine/threonine kinase activator activity

Subcellular Location

Cytoplasm

Structure

Component of trimeric complex composed of STK11/LKB1, STRAD, and CAB39.  Tethers STK11/LKB! In the cytoplasm and stimulates its catalytics activity.

Alternative Nomenclature

CGI 66 antibody

MO25 alpha antibody

General Information

Product

Calpain Antibody

Description

Affinity Purified Calpain Schistosoma Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Reactivity

S. mansoni

Immunogen

Synthetic peptide taken within amino acid region 700-758 on Schistosoma mansoni Calpain protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P27730

Overview

Calcium-activated non-lysosomal thiol-proteases.

Molecular Function

Calcium, Metal-binding.

Subcellular Location

Intracellular

Expression

Sporocysts and adult worms

Structure

Belongs to the peptidase C2 family; 1 Calpain catalytic domain; 2 EF-hand domains

Alternative Nomenclature

Calcium activated neutral proteinase 1 antibody

Calpain 1 (mu/I) large subunit antibody

CANP 1 antibody

Cell proliferation inducing protein 30 antibody

Micromolar Calpain antibody

Mu Calpain antibody

muCANP antibody

muCL antibody

General Information

Product

MAP3K10 Antibody 

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 10 Antibody

Verified Applications

DB, ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide correpsonding to the C-terminal taken within amino acid region 900-950 on human MAP3K10 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02779

Overview

Activates the JUN N-terminal pathway.

Molecular Function

ATP binding; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm

Expression

Expressed in brain and skeletal muscle.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Alternative Nomenclature

MEKK10 antibody

MEKK9 antibody

Mitogen activated protein kinase kinase kinase 10 antibody

Mitogen activated protein kinase kinase kinase 9 antibody

Mixed lineage kinase 2 antibody

MKN28 derived nonreceptor type serine/threonine kinase antibody

MKN28 kinase antibody

MLK1 antibody

MLK2 antibody

MST antibody

PRKE1 antibody

Protein kinase MST antibody

General Information

Product

Eprhin Receptor A4 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 550-650 on human EphA R4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q03137

Overview

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via

Alternative Nomenclature

Cek 8 antibody

EK8 antibody

eph receptor a4 antibody

EPH-like kinase 8 antibody

EphA4 antibody

Ephrin receptor Eph A4 antibody

hEK8 antibody

Receptor protein tyrosine kinase HEK 8 antibody

Sek 1 antibody

SEK antibody

TYRO 1 protein tyrosine kinase antibody

Tyrosine-protein kinase receptor SEK antibody

General Information

Product

Ephrin Receptor A7 Antibody

Description

Affinity Purified EPHA7 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding taken within amino acid region 500-550 on human Ephrin type-A receptor 7 protein.

Accession #

NCBI:  NP_004431

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q15375

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. 

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Single-pass type 1 membrane protein

Expression

Widely Expressed

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (via PDZ-binding motif) with GRIP1 and PICK.

Alternative Nomenclature

Cek 11 

Developmental kinase 1 

EBK 

EHK3 

EK11 

Embryonic brain kinase 

Eph homology kinase 3 

Eph receptor A7 

EPH-like kinase 11 

EphA7 

Ephrin type-A receptor 7 

hEK11 

MDK 1 

Receptor protein tyrosine kinase HEK 11 

Tyrosine protein kinase receptor EHK 3 

General Information

Product

Eprhin Receptor A6 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 6 isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 800-900 on human EphA R6 protein.

Accession #

NCBI:  NP_001073917

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B3KS12

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Expressed in brain and testis.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Ehk 2 antibody

EPH homology kinase 2 antibody

Eph Receptor A6 antibody

ephrin receptor EphA6 antibody

Hek12 antibody