General Information

Product

Cofilin Antibody

Description

Affinity Purified Cofilin-1 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 120-166 on human Cofilin protein.

Accession #

NCBI: BAA00589.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P23528

Overview

Binds to F-actin and exhibits pH-sensitive depolymerizing activity.  Also regulates cytoskeleton dynamics.

Molecular Function

Actin-Binding

Subcellular Location

Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane; Nucleus

Expression

Various tissues

Structure

Contains 1 ADF-H domain and belongs to actin-binding proteins ADF family

Alternative Nomenclature

18 kDa phosphoprotein antibody

CFL 1 antibody

epididymis secretory protein Li 15 antibody

HEL-S-15 antibody

non-muscle isoform antibody

p18 antibody

General Information

Product

EDIL3 Antibody

Description

Affinity Purified EGF-like repeat and discoidin I-like domain-containg protein 3 isoform Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 30-60 on Del-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.69-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

O35474

Overview

Promotes endothelial cells through interaction with the alpha-v/beta-3 integrin receptor.  Inhibits formation of vascular-like structures.

Molecular Function

Calcium, Metal-binding

Subcellular Location

Secreted

Expression

Angioblasts and early endothelial cells

Structure

Contains 3 EGF-like domains; Contains 3 F5/8 type C domains

Alternative Nomenclature

DEL1 antibody

Developmentally regulated endothelial cell locus 1 protein antibody

EGF like repeat and discoidin I like domain containing protein 3 antibody

Integrin-binding protein DEL1 antibody

General Information

Product

Junctional Adhesion Molecule 1 Antibody

Description

Affinity Purified Junctional adhesion molecule A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Monkey, Mouse, Panda, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-299 on human Junctional Adhesion Molecule A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y624

Overview

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3.  Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.

Subcellular Location

Cell junction, cell membrane, single-pass type I membrane protein (Note: Localized at tight junctions of both epithelial and endothelial cells)

Alternative Nomenclature

9130004G24 antibody

AA638916 antibody

CD 321 antibody

ESTM33 antibody

F11 receptor antibody

F11R antibody

JAM 1 antibody

JAM A antibody

JCAM antibody

Junction adhesion molecule 1 antibody

KAT antibody

Ly106 antibody

PAM1 antibody

Platelet adhesion molecule 1 antibody

Platelet F11 receptor antibody

PRO301 antibody

UNQ264/PRO301 antibody

General Information

Product

PAR3 Antibody

Description

Affinity Purified Proteinase-activated receptor 3 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on mouse Proteinase-activated receptor 3 protein.

Accession #

NCBI: NP_032001.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:12,000

ELISA

1:12,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:5,000

Protein

Uniprot #

O88634

Overview

Functions as a receptor for activated thrombin or trypsin coupled to G-proteins that stimulate phosphoinositide hydrolysis.  May also function in contributing to the activation of platelets.

Molecular Function

G-protein coupled receptor, Receptor, Transducer, thrombin receptor activity

Alternative Nomenclature

agouti signaling protein antibody

ASIP antibody

Atypical PKC isotype specific interacting protein antibody

Baz antibody

bazooka antibody

Coagulation factor II receptor-like 2 antibody

CTCL tumor antigen se2-5 antibody

F2rl2 antibody

PAR-3 antibody

PAR3-alpha antibody

PARD-3 antibody

Partitioning-defective protein 3, C. elegans, homolog of antibody

SE2-5L16 antibody

SE2-5LT1 antibody

SE2-5T2 antibody

Thrombin receptor-like 2 antibody

General Information

Product

Synaptoagmin 1 Antibody Affinity Purified

Description

Affinity Purified SYT1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic  peptide taken within amino acid region 1-60 on SYT1 protein.

Accession #

GenBank: AAH58917.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P21579

Overview

May have regulatory rol in membrane interactions during trafficking of synaptic vesicles at active zones of synapse. Binds acidic phospholipids, can bind to at least three additional proteins in a CA2+ independent manner.

Molecular Function

1-phosphatidylinositol binding, calcium dependent phospholipid binding, calcium ion binding, low-density lipoprotein particle receptor binding, phosphatidylserine binding, transporter activity

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, single-pass membrane protein (Note: Synaptic vesicles and chromaffin granules.)

Alternative Nomenclature

P65 antibody

SVP65 antibody

Synaptotagmin 2 antibody

Synaptotagmin 1 antibody

SYT antibody

SYT1 antibody

Syt2 antibody

SytI antibody

General Information

Product

TYRO3 Antibody

Description

Affinity Purified Tyrosine-protein kinase receptor TYRO3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid reigon 800-850 on human Tyrosine-protein kinase receptor TYRO3 protein.

Accession #

NCBI:  NP_006284.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q06418

Overview

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass Type I membrane protein

Expression

Abundant in the brain and lower levels in other tissues.

Structure

Monomer and homodimer. Interacts (via N-terminus) with extracellular ligands TULP1 and GAS6. Interacts with PIK3R1; this interaction increases PI3-kinase activity.

Alternative Nomenclature

Brt 

BYK 

DTK 

RSE 

SKY 

Tif 

TYRO3 

Tyrosine-protein kinase byk 

Tyrosine-protein kinase DTK 

Tyrosine-protein kinase receptor TYRO3 

Tyrosine-protein kinase RSE 

Tyrosine-protein kinase SKY 

General Information

Product

CXCR7 Antibody

Description

Affinity Purified Chemokine (C-X-C motif) receptor 7 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human CXCR7 protein.

Accession #

GenBank: AAH36661

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P25106

Overview

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival.

Molecular Function

Cell adhesion, Host-virus interaction

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Early endosome. Recycling endosome

Expression

Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue.

Structure

Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity. Interacts with ARRB1 and ARRB2.

Alternative Nomenclature

ACKR3 antibody

atypical chemokine receptor 3 antibody

C X C chemokine receptor type 7 antibody

Chemokine orphan receptor 1 antibody

CMKOR1 antibody

CXC R7 antibody

G protein coupled receptor 159 antibody

G protein coupled receptor RDC1 homolog antibody

GPR159 antibody

GPRN1 antibody

RDC 1 antibody

General Information

Product

FAM38A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken between TMD14 and TMD15 on human FAM38A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q92508

Overview

Pore-forming subunit of a mechanosensitive non-specific cation channel;positive regulation of cell-cell adhesion mediated by integrin

Molecular Function

Ion channel

Subcellular Location

Cell membrane, Endoplasmic reticulum, Membrane

Expression

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes;Expressed in erythrocytes

Structure

Homooligomer, most likely homotetramer

Alternative Nomenclature

DHS antibody

Fam38a antibody

Family with sequence similarity 38 member A antibody

KIAA0233 antibody

Membrane protein induced by beta-amyloid treatment antibody

Mib antibody

Piezo-type mechanosensitive ion channel component 1 antibody

Protein FAM38A antibody

Protein FAM38B antibody

Protein PIEZO1 antibody

General Information

Product

GPR56 Extracellular domain Antibody

Description

Affinity Purified G-protein coupled receptor 56 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken from large extracellular loop of the human GPCR56 protein. Peptide was conjugated to Keyhole Limpet Haemocyanin (KLH).

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9Y653

Overview

Involved in cell adhesion and probably in cell-cell interactions.

Molecular Function

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Membrane, Secreted

Expression

highest levels found in thyroid gland, brain and heart;Expressed in a great number of tumor cells.

Structure

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily

Alternative Nomenclature

7 transmembrane protein with no EGF like N terminal domains 1 antibody

BFPP antibody

DKFZp781L1398 antibody

EGF TM7 like antibody

G protein coupled receptor 56 antibody

Gpr56 antibody

Polymicrogyria bilateral frontoparietal antibody

Protein TM7XN1 antibody

TM7LN4 antibody

TM7XN1 protein antibody

General Information

Product

MACF1 Antibody

Description

Affinity Purified Microtuble-Actin crosslinking factor 1 Antibody

Verified Applications

ELISA, ICC, IFC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Recombinant protein

Accession #

GenBank: CAM18553.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9QXZO

Overview

F-actin bidning protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds microtubles.  Acts as positive regulator of Wnt receptor signaling pathway and is invovled in translocation of AXIN12 and its associated complex from the cytoplasm to cell membrane.  Has actin-regulated ATPase activity and is inovled in focal adhesios assembly and dynamics.

Molecular Function

Actin binding, calcium ion binding, ATPase activity, microtuble binding

Subcellular Location

Hair follicle stem cells; Ubiquitous expression; lung; heart; thymus; spleen; brain.

Expression

Expressed in neurontal tissues and foregut of embryo

Structure

Complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Alternative Nomenclature

ABP620 antibody

Acf7 antibody

Aclp7 antibody

Actin cross linking family 7 antibody

MACF antibody

Microtubule actin crosslinking factor 1 antibody

rabeculin alpha antibody