General Information

Product

Phospho-STAT3 Antibody

Description

Affinity Purified Phosphorylated Signal transducer and activator of transcription Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 700-750 on human STAT3 protein.

Accession #

NCBI:  NP_644805

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P40763

Overview

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.

Molecular Function

Activator, calcium ion binding, DNA binding

Subcellular Location

Cytoplasm. Nucleus

Expression

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Structure

Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression (By similarity).

Alternative Nomenclature

Acute Phase Response Factor antibody

APRF antibody

DNA binding protein APRF antibody

HIES antibody

Signal Transductor and Activator of Transcription 3 antibody

General Information

Product

STAT3 Antibody

Description

Affinity Purified Signal transducer and activator of transcription Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human STAT3 protein.

Accession #

NCBI:  NP_644805

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40763

Overview

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.

Molecular Function

Activator, calcium ion binding, DNA binding

Subcellular Location

Cytoplasm. Nucleus

Expression

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Structure

Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. 

Alternative Nomenclature

Acute Phase Response Factor antibody

APRF antibody

DNA binding protein APRF antibody

HIES antibody

Signal transducer and activator of transcription 3 (acute phase response factor) antibody

STAT 3 antibody

General Information

Product

Phospho Protein Vestigial Antibody Affinity Purified

Description

Affinity Purified Vestigial Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Vestigial protein.

Accession #

NCBI:  NP_523723.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q26366

Overview

Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.

Molecular Function

DNA binding; Protein C-terminus binding

Subcellular Location

Nucleus

Expression

Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.

Alternative Nomenclature

VG

VSTGL

VG21

DMEL/CG3830

CCG3830

General Information

Product

REST / NRSF Antibody Affinity Purified

Description

Affinity Purified RE1-silencing transcription factor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1097 on human REST protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.54 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13127

Overview

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes.

Molecular Function

Repressor, Chromatin binding, Core promoter binding

Subcellular Location

Nucleus

Expression

Ubiquitous, expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovaries.

Structure

Interacts with SIN3A, SIN3B and RCOR1.  Interacts with CDYL.

Alternative Nomenclature

Neural Restrictive Silencer Factor antibody

NRSF antibody

RE1 silencing transcription factor antibody

Repressor binding to the X2 box antibody

REST antibody

X2 box repressor antibody

XBR antibody

General Information

Product

RUNX1 / AML1 Antibody Affinity Purified

Description

Affinity Purified RUNX1 Protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-80 on human RUNX1 protein.

Accession #

GenBank: AAI36381.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q01196

Overview

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis.

Molecular Function

ATP binding, DNA binding, calcium ion binding

Subcellular Location

Nucleus

Expression

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Structure

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize.

Alternative Nomenclature

Acute myeloid leukemia 1 protein antibody

alpha subunit core binding factor antibody

AML1 EVI 1 fusion protein antibody

Aml1 oncogene antibody

AMLCR 1 antibody

CBF alpha 2 antibody

Core binding factor runt domain alpha subunit 2 antibody

Core-binding factor subunit alpha-2 antibody

EVI1 antibody

HGNC antibody

Oncogene AML 1 antibody

PEBP2 alpha B antibody

Polyomavirus enhancer binding protein 2 alpha B subunit antibody

Run1 antibody

Runt-related transcription factor 1 antibody

SL3/AKV core binding factor alpha B subunit antibody

General Information

Product

RUNX2 Antibody

Description

Affinity Purified Runt-related transcription factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda

Immunogen

Synthetic peptide taken within amino acid region 10-50 on human RUNX2 protein.

Accession #

NCBI:  XP_001100855.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0000D9AD12

Overview

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.

Molecular Function

DNA-binding; Transcription Regulation

Subcellular Location

Nucleus

Structure

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with HIVEP3. Interacts with IFI204. I

Alternative Nomenclature

Acute myeloid leukemia 3 protein antibody

Alpha subunit 1 antibody

AML3 antibody

CBF alpha 1 antibody

CBFA1 antibody

CCD1 antibody

Cleidocranial dysplasia 1 antibody

Core binding factor runt domain alpha subunit 1 antibody

Core binding factor subunit alpha 1 antibody

Oncogene AML 3 antibody

OSF 2 antibody

Osteoblast specific transcription factor 2 antibody

PEBP2 alpha A antibody

PEBP2A1 antibody

PEBP2A2 antibody

Polyomavirus enhancer-binding protein 2 alpha A subunit antibody

Runt domain antibody

Runt related transcription factor 2 antibody SL3 3 enhancer factor 1 alpha A subunit antibody

General Information

Product

SFRP2 Antibody 

Description

Affinity Purified Secreted frizzled-related protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 225-295 on human SFRP2.

Accession #

GenBank: AAH08666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96HF1

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Molecular Function

Endopeptidase activator activity, integrin binding, PDZ domain binding, Wnt-activated receptor activity, fibronectin binding, receptor agonist activity, Wnt-protein binding

Subcellular Location

Secreted

Expression

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Alternative Nomenclature

AI851596 antibody
Frizzled related protein 2 antibody
FRP2 antibody
SARP1 antibody
SDF5 antibody
Secreted apoptosis related protein 1 antibody
Secreted frizzled-related protein 2 antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 340-390 on human SIRT7 protein.

Accession #

GenBank: AAH17305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NRC8

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody

General Information

Product

SREBP2 Antibody

Description

Affinity Purified Sterol regulatory element binding protein-2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.

Accession #

GenBank: AAA50746.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q12772

Overview

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

Molecular Function

Activator, E-box binding, protein C-terminus binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein, Golgi apparatus membrane; Multi-pass membrane protein, Cytoplasmic vesicle > COPII-coated vesicle membrane; Multi-pass membrane protein

Expression

Ubiquitously expressed in adult and fetal tissues.

Structure

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF13.

Alternative Nomenclature

AI608257 antibody

bHLHd2 antibody

Class D basic helix-loop-helix protein 2 antibody

Processed sterol regulatory element-binding protein 2 antibody

SREBF 2 antibody

SREBP2 antibody

Sterol regulatory element-binding transcription factor 2 antibody

General Information

Product

ETS1 associated protein II Antibody

Description

Affinity Purified tyrosyl-DNA phosphodiesterase 2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-360 on human Tyrosyl-DNA phosphodiesterase protein.

Accession #

NCBI:  NP_057698.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

O95551

Overview

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue.

Molecular Function

5'-tyrosyl-DNA phosphodieseterase activity, magnesium ion binding, manganese ion binding, nuclease activity, transcription corepressor activity, single-stranded DNA binding

Subcellular Location

nucleus, PML body, cytoplasm

Expression

Widely expressed

Alternative Nomenclature

5'-tyrosyl-DNA phosphodiesterase antibody

AD 022 antibody

dJ30M3.3 antibody

EAP 2 antibody

ETS 1 associated protein 2 antibody

RP1-30M3.3 antibody

TRAF and TNF receptor associated protein antibody

TTRAP antibody

Tyr DNA phosphodiesterase 2 antibody