General Information

Product

Eprhin Receptor B4 Antibody

Description

Affinity Purified Ephrin type-B receptor 4 precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 900-984 on human Ephrin B4 receptor protein.

Accession #

NCBI:  NP_004435

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54760

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell Membrane

Expression

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.

Alternative Nomenclature

Ephb4 antibody

Ephrin type-B receptor 4 antibody

Hepatoma Transmembrane Kinase antibody

HTK antibody

MYK 1 antibody

MYK1 antibody

TYRO 11 antibody

Tyrosine-protein kinase receptor HTK antibody

Tyrosine-protein kinase TYRO11 antibody

General Information

Product

Eprhin Receptor B3 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 3 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein.

Accession #

NCBI:  NP_034273

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

P54754

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level).

Structure

Contains 1 Eph LBD domain; Contains 2 fibronectin type-III domains; Contains 1 protein kinase domain; Contains 1 SAM domain

Alternative Nomenclature

Cek10 antibody

EK2 antibody

Embryonic kinase 2 antibody

EPH Like Tyrosine Kinase 2 antibody

EPH receptor B3 antibody

EPH-like kinase 2 antibody

ephb3 antibody

Ephrin type-B receptor 3 antibody

ETK2 antibody

hEK2 antibody

Human Embryo Kinase 2 antibody

Mdk5 antibody

Sek4 antibody

TYRO6 antibody

Tyrosine protein kinase receptor HEK2 antibody

Tyrosine-protein kinase TYRO6 antibody

General Information

Product

Eprhin Receptor A8 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-A receptor 8 isoform 1 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on human EphA R8 protein.

Accession #

NCBI:  NP_065387

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein; Early endosome membrane

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. May also form heterodimers with other ephrin receptors (By similarity).

Alternative Nomenclature

2.7.1.112 antibody

EEK antibody

EK3 antibody

EPH and ELK related kinase antibody

EPH like kinase 3 antibody

EphA8 antibody

Ephrin type-A receptor 8 antibody

HEK3 antibody

KIAA1459 antibody

Tyrosine-protein kinase receptor EEK antibody

Tyrosylprotein Kinase antibody

General Information

Product

Ephrin Receptor A3 Antibody

Description

Affinity Purified Ephrin Type-A Receptor 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-980 on human EPHA3 protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P29320

Overview

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Isoform 1:  Cell membrane; Single-pass type I membrane protein.  Isoform 2:  Secreted

Expression

Widely expressed. Highest level in placenta.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding.

Alternative Nomenclature

AW492086 antibody

Cek4 antibody

EC 2.7.10.1 antibody

EK4 antibody

End3 antibody

Eph receptor A3 antibody

EPH-like kinase 4 antibody

EPH-like tyrosine kinase 1 antibody

EphA3 antibody

Ephrin type-A receptor 3 antibody

ETK 1 antibody

ETK antibody

HEK 4 antibody

HEK antibody

Human embryo kinase 1 antibody

Mek4 antibody

MGC109882 antibody

Receptor tyrosine kinase HEK antibody

Tyro 4 antibody

Tyrosine-protein kinase TYRO4 antibody

General Information

Product

ACK1 Antibody

Description

Affinity Purified Activated CDC42 kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein.

Accession #

GenBank: L13738.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q07912

Overview

Non-receptor tyrosine-protein and serine/threonine-protein kinase implicated in cell sperading and migration, survival, growth and proliferation.  Transduces extracellular signals to cytosolic and nuclear effectors.

Molecular Function

ATP binding; epidermal growth factor receptor binding; GTPase inhibitor activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine kinase activity

Subcellular Location

Cell membrane; Nucleus; Endosome; Cell junction; adherens junction; Cytoplasmic vessicle membrane; Peripheral membrane protein; Clathrin-coated vessicles; Clathin coated pit

Expression

During progressive stages in breast and prostate cancer

Structure

Forms  complexes with GRB2 and several receptor tyrosine kinases.  Part of collagen stimulated complex.

Alternative Nomenclature

Acetate kinase 1 antibody

Acetokinase 1 antibody

ACK 1 antibody

Activated Cdc42 associated kinase 1 antibody

Activated CDC42 kinase 1 antibody

Activated p21cdc42Hs kinase antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non-receptor protein 2 antibody

General Information

Product

Axl Antibody

Description

Affinity Purified AXL receptor tyrosine kinase UFO isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 840-880 on mouse AXL protein. The peptide was post translationally modified to acheive proper orientation and antigenicity.

Accession #

NCBI:  NP_033491

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q00993

Overview

Receptor tyrosine kinase that transduces signals from the ECM into cytoplasm by binding growth factor GAS6. Regulates survival, proliferation, migration and differentiation.

Molecular Function

ATP-binding, Nucleotide binding, Kinase, Receptor, Transferase, Tyrosine-protein kinase, Immunity Differentation and Innate Immunity.

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Expression

Developing tissues in late embryogenesis. In cells forming organ capsules as well as in connective tissue structures in adult.

Structure

Heterodimer and heterotetramer with ligand GAS6.

Alternative Nomenclature

Adhesion related kinase 

Ark 

Tyro7 

Tyrosine protein kinase receptor UFO 

General Information

Product

BTK Antibody

Description

Affinity Purified Bruton's Tyrosine Kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-650 on human BTK protein.

Accession #

NCBI: NP_000052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q06187

Overview

Non-receptor tyrosine kinase invovled in B lymphocyte development.

Molecular Function

Kinase; Transferase; Tyrosine-protein Kinase

Subcellular Location

Cytoplasm; Cell Membrane; Peripheral membrane: Nucleus.  In steady state BTK is predominantly cytosolic.

Expression

B-lymphocytes

Structure

PH domain mediates binding to inositol polyphosphate and phosphoinositides, leading to its targeting with plasma membrane.  Extended in the BTK kinase family by a region designated Tec homology domain, which has roughly 80 residues preceding SH3 domain.

Alternative Nomenclature

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

General Information

Product

CCK4 / PTK7 Antibody

Description

Affinity Purified Protein Tyrosine Kinase 7 Antibody

Verified Applications

ELISA, ICC/IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Purified Protein tyrosine kinase 7 protein was emulsified in complete adjuvant and used to immunize the rabbits. 

Accession #

NCBI:  AAH50050

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal. The anti-serum was pre-cleared for tag protein and then affinity purified on immobilized PTK7 affinity chromatography.

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q12852

Overview

Activator of JNK/SAPK pathway.  Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Molecular Function

ATP-binding, magnesium, Nucleotide-binding.

Subcellular Location

Cytoplasm, Membrane, Peripheral membrane protein

Expression

Highly expressed in brain and kidney.

Structure

Interacts with MBIP.

Alternative Nomenclature

Colon carcinoma kinase 4 antibody

Inactive tyrosine-protein kinase 7 antibody

mPTK7/CCK4 antibody

Serum response factor antibody

Srf antibody

General Information

Product

ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 isoform b Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 130-180 receptor tyrosine-protein kinase erbB-2 protein.

Accession #

NCBI:  NP_001005862.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by hereguline (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

c erb B2 antibody

CD340 antibody

cneu antibody

Erb B2 antibody

Erythroblastosis Virus antibody

HER 2 antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN 19 antibody

NEU proto oncogene antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Proto-oncogene Neu antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

TKR1 antibody

verbb2 antibody

General Information

Product

ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 isoform a Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 1125-1175 receptor tyrosine-protein kinase erbB-2 protein.

Accession #

NCBI:  NP_004439.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Determinant

N-epitope

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

CD340 antibody

CerbB2 antibody

cneu antibody

Erb B2 antibody

erbb2 antibody

Erythroblastosis Virus antibody

HER 2 antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN 19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Proto-oncogene Neu antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody