General Information

Product

Adiponectin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig

Immunogen

Synthetic peptide taken within amino acid region 150-243 on pig Adiponectin protein.

Accession #

GenBank: AY589691

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.65 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q69907

Overview

Involved in brown fat cell differentiation, responses to drugs, detection of oxidative stress glucose homeostasis and fatty acid oxidation.  Invovled in regulation of DNA biosynthetic processes.

Molecular Function

Hormone activity; receptor binding

Subcellular Location

Cell periphery; collagen trimer; extracellular space; cell surface; endoplasmic reticulum; perinuclear region of cytoplasm

Expression

Secreted

Alternative Nomenclature

30 kDa adipocyte complement related protein antibody

ACDC antibody

ACRP 30 antibody

Adipocyte antibody

Adipocyte C1q and collagen domain containing protein antibody

Adipocyte complement related 30 kDa protein antibody

adipocyte-specific secretory protein antibody

Adiponectin antibody

adiponectin, C1Q and collagen domain containing antibody

AdipoQ antibody

Adipose most abundant gene transcript 1 antibody

Adipose specific collagen like factor antibody

ADIPQTL1 antibody

ADPN antibody

APM 1 antibody

C1q and collagen domain-containing protein antibody

GBP 28 antibody

Gelatin binding protein antibody

Gelatin binding protein 28 antibody

General Information

Product

FFAR3 Antibody

Description

Affinity Purified Free fatty acid receptor A3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 270-320  on human FFAR3 protein.

Accession #

NCBI:  NP_005295.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O14843

Overview

G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation (PubMed:12711604). 

Molecular Function

G-protein coupled receptor activity; Lipid binding

Subcellular Location

Cell Membrane; Multi-Pass Membrane Protein

Expression

Highest level in adipose tissue, and lower expression across all tissues tested. Expressed in sympathetic ganglia.

Structure

Belongs to the G-protein coupled receptor 1 family.

Alternative Nomenclature

FFA3R antibody

Free fatty acid receptor 3 antibody

G protein coupled receptor 41 antibody

GPCR41 antibody

GPR41 antibody

gpr42 antibody

General Information

Product

GAPDH Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 75-150 on mouse Glyceraldehyde-3-phosphate dehydrogenase protein.

Accession #

NCBI:  NP_032110.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P16858

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively.

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain; cerebullum; heart; kidney; liver; skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody

General Information

Product

IFI16 Antibody Affinity Purified

Description

Affinity Purified Gamma-interferon-inducible protein 16 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 140-190 on human IFI16 protein

Accession #

NCBI:  NP_001193496.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q16666

Overview

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein.

Molecular Function

Core promoter binding, identical protein binding, transcription factor binding, double-standard DNA binding, poly(A) RNA binding

Subcellular Location

Nucleus, cytoplasm (Note: Cellular distribution is dependent on acetylation status of multipartite nuclear localization signal(NLS); NLS acetylation promotes cytoplasmic localization. Localizes in nucleus during human herbes simplex virus 1 infection)

Expression

Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prosta

Alternative Nomenclature

IFI16 antibody

Gamma interferon inducible protein 16 antibody

Ifi204 antibody

IFNGIP1 antibody

interferon activated gene 204 antibody

Interferon gamma inducible protein 16 antibody

p204 antibody

PYHIN2 antibody

General Information

Product

LOXL1 Antibody

Description

Affinity Purified Loxl1 protein Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 570-607 on rat LOXL1 protein.

Accession #

GenBank: AAH03973.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P97873

Overview

Active on elastin and collagen substrates.

Molecular Function

Copper ion binding, oxidereductase activity acting on the CH-NH2 group of donors and oxygen as accecptor

Subcellular Location

secreted, extracellular space

Alternative Nomenclature

Lysyl oxidase like 1 antibody

LOL antibody

Lysyl oxidase homolog 1 antibody

Lysyl oxidase like protein 1 antibody

General Information

Product

Niemann Pick C1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1228-1278 on human Niemann-Pick C1 protein.

Accession #

NCBI:  NP_000262.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:4,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15118

Overview

Intracellular cholesterol transporter plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment.

Molecular Function

Receptor activity

Subcellular Location

Endosome, Lysosome, Membrane

Expression

Adrenal gland; Cerebral cortex; lung; placenta; lymph node

Structure

Belongs to the patched family.

Alternative Nomenclature

Niemann Pick C1 antibody

Niemann Pick C1 protein precursor antibody

Niemann Pick disease, type C1 antibody

NPC antibody

NPC1 antibody

General Information

Product

PDE5A Antibody

Description

Affinity Purified cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC-P, IHC-Fr, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig, Rat

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:1,000

Protein

Uniprot #

O76074

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific 3'5' cyclic phosphodiesterase antibody

cGMP specific phosphodiesterase PDE5A2 antibody

cGMP specific phosphodiesterase type 5A antibody

cGMP-specific 3'' antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A1 antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

Phospho-PDE5A Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Pig, Rat, Sheep

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted 

Accession #

GenBank: EAX05277.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.0-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0000EE2B22

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific phosphodiesterase type 5A antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A1 antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

SESN1 Antibody

Description

Affinity Purified Sestrin-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human SESN1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y6P5

Overview

Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth.

Molecular Function

Cell cycle arrest; Cellular response to DNA damage stimulus; Gene expression; Regulation of protein kinase B signaling; Transcription initation from RNA polymerase II promoter; Regulation of response to reactive oxygen species; Negative regulation of cell.

Subcellular Location

Nucleus

Expression

Widely Expressed

Structure

Belongs to the Sestrin family.

Alternative Nomenclature

1110002G11Rik antibody

AU044290 antibody

p53 activated gene 26 antibody

p53 regulated PA26 nuclear protein antibody

PA26 antibody

RP11-787I22.1 antibody

sesn1 antibody

SEST1 antibody

Sestrin-1 antibody

General Information

Product

SESN2 Antibody

Description

Affinity Purified Sestrin 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-80 on SESN2 protein.

Accession #

GenBank: AAM92261.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P58004

Overview

Involved in the reduction of peroxiredoxins.

Subcellular Location

Cytoplasm, Nucleus

Expression

Widely expressed.

Alternative Nomenclature

Hi95 antibody

Hypoxia induced gene 95 antibody

RP23 63D8.3 antibody

SES2 antibody

SEST2 antibody