General Information

Product

AKT1 Antibody

Description

Affinity Purified RAC-alpha serine/threonine-protein kinase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within the last 50 amino acid on human RAC-alpha serine/threonine-protein kinase protein.

Accession #

GeneBank: AAA36539

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

PKB antibody

Protein Kinase B Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

General Information

Product

BTK Antibody

Description

Affinity Purified Bruton's Tyrosine Kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-650 on human BTK protein.

Accession #

NCBI: NP_000052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q06187

Overview

Non-receptor tyrosine kinase invovled in B lymphocyte development.

Molecular Function

Kinase; Transferase; Tyrosine-protein Kinase

Subcellular Location

Cytoplasm; Cell Membrane; Peripheral membrane: Nucleus.  In steady state BTK is predominantly cytosolic.

Expression

B-lymphocytes

Structure

PH domain mediates binding to inositol polyphosphate and phosphoinositides, leading to its targeting with plasma membrane.  Extended in the BTK kinase family by a region designated Tec homology domain, which has roughly 80 residues preceding SH3 domain.

Alternative Nomenclature

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

General Information

Product

ITK Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-600 on human ITK protein.

Accession #

GenBank: BAA02873.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q08881

Overview

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.

Molecular Function

ATP binding, metal ion binding, non-membrane spanning protein tyrosine kinase activity

Subcellular Location

Cytoplasm (Note: Localizes in vicinity of cell surface receptors in plasma membrane after receptor stimulation)

Expression

T-cell lines and natural killer cell lines.

Alternative Nomenclature

EMT antibody

Homolog of mouse T cell itk/tsk antibody

IL 2 inducible T cell kinase antibody

Itk antibody

Kinase EMT antibody

LPFS1 antibody

LYK antibody

MGC126257 antibody

MGC126258 antibody

PSCTK 2 antibody

T cell specific kinase antibody

TSK antibody

Tyrosine protein kinase antibody

Tyrosine protein kinase ITK/TSK antibody

General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody

General Information

Product

LIM kinase 2 Antibody

Description

Affinity Purified Lysine-specific demethylase 4C Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 570-638 on human LIMK2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53671

Overview

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code

Molecular Function

Androgen receptor binding, enzyme binding, histone demethylase activity, zinc ion binding, dioxygenase activity

Subcellular Location

Nucleus

Expression

Overexpressed in several esophageal squamous cell carcinomas (ESCs).

Alternative Nomenclature

LIM domain kinase 2 antibody

LIMK 2 antibody

General Information

Product

MAP3K12 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 12 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 270-320 on MAP3K12 protein.

Accession #

GenBank: AAH50050.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q12852

Overview

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Molecular Function

ATP Binding, MAP kinase kinase kinase activity, protein kinase binding

Subcellular Location

Cytoplasm, membrane, peripheral membrane protein

Expression

Highly expressed in brain and kidney.

Alternative Nomenclature

DLK antibody

Dual leucine zipper bearing kinase antibody

Leucine-zipper protein kinase antibody

Map3k12 antibody

MAPK-upstream kinase antibody

MEKK12 antibody

Mixed lineage kinase antibody

MUK antibody

ZPK antibody

ZPKP1 antibody

General Information

Product

MAP3K14 Antibody Affinity Purified

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 14 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-350 on human MAP3K14 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q99558

Overview

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Alternative Nomenclature

MAP3K14 antibody

FTDCR1B antibody

HS antibody

HsNIK antibody

Mitogen activated protein kinase kinase kinase 14 antibody

NF kappa beta inducing kinase antibody

NIK antibody

Serine/threonine-protein kinase NIK antibody

General Information

Product

MAP3K8 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 8 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-467 on human MAP3K8 protein.

Accession #

GenBank: AAI13567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P41279

Overview

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Expressed in several normal tissues and human tumor-derived cell lines.

Structure

Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KRS2; the interaction inhibits ERK and NF-kappa-B activation.

Alternative Nomenclature

c COT antibody

Cancer Osaka thyroid oncogene antibody

COT antibody

COT proto oncogene serine/threonine protein kinase antibody

EST antibody

ESTF antibody

Ewing sarcoma transformant antibody

FLJ10486 antibody

MEKK8 antibody

Mitogen activated protein kinase kinase kinase 8 antibody

Proto oncogene cCot antibody

Serine/threonine-protein kinase cot antibody

TPL 2 antibody

Tumor progression locus 2 antibody

General Information

Product

mTOR Antibody Affinity Purified

Description

Affinity Purified Serine/threonine-protein kinase mTOR Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-480 on mTOR protein.

Accession #

NCBI:  NP_063971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42346

Overview

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Alternative Nomenclature

FK506 binding protein 12 rapamycin associated protein 1 antibody

FKBP rapamycin associated protein antibody

FRAP1 antibody

FRAP2 antibody

mTOR antibody

RAFT1 antibody

Rapamycin target protein antibody

RAPT1 antibody

Serine/threonine-protein kinase mTOR antibody

General Information

Product

Phospho-ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 (HER2) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Phoshporylated synthetic peptide taken within amino acid region 1100-1150 on human HER-2 protein

Accession #

GenBank:  AAA75493.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

c erb B2/neu protein antibody

CD340 antibody

CerbB2 antibody

Erb B2 antibody

Erythroblastosis Virus antibody

HER 2/neu antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

TKR1 antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody

verbb2 antibody