General Information

Product

Lin28 Antibody

Description

Affinity Purified LIN28 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid sequence 1-60 on Lin28A sequence.

Accession #

GenBank: AAH28566.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q9H9Z2

Overview

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Association with translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing mRNA's

Molecular Function

DNA binding, mRNA binding, zinc ion binding, miRNA binding, RNA binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: nucleolar localization observed in 10-15% of nuclei in differentiated byotubes. Shuttles between cytoplasm and nucleus. Localizes to cytoplasmic processing bodies and stress granules)

Expression

Expressed in embryonic stem cells (ES cells), placenta and testis.

Alternative Nomenclature

AL024421 antibody

CSDD1 antibody

FLJ12457 antibody

Lin 28 antibody

Protein lin-28 homolog A antibody

RNA binding protein lin 28 antibody

Tex17 antibody

ZCCHC1 antibody

Zinc finger CCHC domain containing 1 antibody

General Information

Product

RASGRF2 Antibody

Description

Affinity Purified Ras-specific guanine nucleotide-releasing factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 1100-1200 on human RASGRF2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O14827

Overview

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP.  Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation.

Molecular Function

Guanine-nucleotide releasing factor, Rho guayl-nucleotide exchange factor activity

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Endoplasmic reticulum membrane; Peripheral membrane protein

Expression

Widely expressed with higher expression in the brain, followed by heart, lung, pancreas and kidney.  Detected in placenta.  Expressed in brain and lung.

Structure

Homooligomer and heterooligomer with RASGRF1.  Interacts with Ras and RAC1.  Interacts in a calcium-dependent manner with calmodulin.

Alternative Nomenclature

GRF2 antibody

Ras guanine nucleotide exchange factor 2 antibody

Ras-GRF2 antibody

General Information

Product

RIC8A Antibody Affinity Purified

Description

Affinity Purified Synembryn-A isoform 1 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acids 500-535 on RIC8A protein

Accession #

NCBI:  NP_068751.4

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q9NPQ8

Overview

Guanine nucleotide exchange factor (GEF) which can activate some G-alpha proteins.  Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP.

Molecular Function

guanyl-nucleotide exchange factor activity, Guanine-nucleotide releasing factor

Subcellular Location

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity.  Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.

Alternative Nomenclature

Protein Ric-8A antibody

resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody

RIC8 antibody

Synembryn-A antibody

General Information

Product

RICH2 Antibody

Description

Affinity Purified Rho GTPase-activating protein 44 isoform 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N terminal amino acids 1-60 of Mouse RICH2.

Sequence:

MKKQFNRMRQ LANQTVGRA E KTEVLSED LL QVEKRLE LVK QVSHST HKKL TACLQ GQQGA

Accession #

NCBI:  NP_778168.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250-1:500

Protein

Uniprot #

Q5SSM3

Overview

GTPase-activating protein (GAP) that stimulates the GTPase activity of the Rho-type GTPases.  Thereby controlling the Rho-type GTPases cycling between their active GTP-bound and inactive GDP bound states.

Molecular Function

GTPase activator activity, phospholipid binding, GTPase activation

Subcellular Location

Cell projection, Recycling endosome, Cell junction

Expression

Expressed in hippocampal neurons at protein level

Structure

Interacts with BST2, SHANK3.

Alternative Nomenclature

KIAA0672 antibody

NPC A 10 antibody

Rho GTPase activating protein RICH2 antibody

RhoGAP interacting with CIP4 homologs protein 2 antibody

General Information

Product

SEPT5 Antibody

Description

Affinity Purified Septin-5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on SEPT5 protein.

Accession #

NCBI:  NP_002679.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q99719

Overview

Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion.

Molecular Function

GTPase activity, structural molecule activity, GTP binding

Subcellular Location

Cytoplasm, cytoskeleton. (Note: In platelets, found in areas surrounding alpha-granules)

Expression

Expressed at high levels in the CNS, as well as in heart and platelets (at protein level).

Alternative Nomenclature

5 Sep antibody

CDCREL1 antibody

Cell division control related protein 1 antibody

H5 antibody

HCDCREL 1 antibody

Peanut like 1 homolog antibody

Peanut-like protein 1 antibody

Platelet glycoprotein Ib beta chain antibody

PNUTL1 antibody

SEPT5 antibody

Septin 5 antibody

General Information

Product

VPS26 Antibody

Description

Affinity Purified Vacuolar protein sorting-associated protein 26A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acids 315-375 on human VPS26 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O75436

Overview

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.

Molecular Function

protein transporter activity

Subcellular Location

cytoplasm, endosome membrane, preipheral membrane protein (Note: Localizes to tubular profiles adjacent to endosomes. Predominantly found in early not late endosomes)

Expression

Down-regulated in Alzheimer disease. No polymorphism or variant is however associated with Alzheimer disease for VPS26A.

Alternative Nomenclature

FLJ12930 antibody

FLJ12930 antibody

HB 58 antibody

Hbeta 58 antibody

Hbeta 58 homolog antibody

MGC94168 antibody

PEP 8A antibody

Vacuolar protein sorting 26 A antibody

Vacuolar protein sorting 26 homolog A yeast antibody

Vacuolar protein sorting associated protein 26A antibody

Vesicle protein sorting 26A antibody

VPS 26 antibody

VPS 26A antibody

General Information

Product

Phospho Chimaerin beta 2 Antibody

Description

Affinity Purified Phosphorylated N-Chimaerin isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequence on beta 2 Chimaerin protein.

Accession #

NCBI: NP_001813

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P15882

Overview

GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.

Molecular Function

GTPase activation

Subcellular Location

Cytosol

Expression

In neurons in brain regions that are involved in learning and memory processes.

Structure

Interacts with EPHA4; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation.

Alternative Nomenclature

A-chimaerin antibody

ARHGAP2 antibody

CHN antibody

Chn1 antibody

N chimaerin antibody

NC antibody

Rho GTPase-activating protein 2 antibody

RHOGAP2 antibody

General Information

Product

PDE1C Antibody

Description

Affinity Purified Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A) Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on PDE1C gene. The sequence was taken within amino acid region 1-50.

Accession #

GenBank: U40371.1 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:40-1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:40-1:100

Immunofluorescence

1:100

Immunohistochemistry

1:40-1:100

Immunoprecipitation

1:200

Western Blot

1:500-1:2,000

Protein

Uniprot #

Q14123

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.

Molecular Function

Hydrolase, calmodulin-dependent cyclic-nucleotide phosphodiesterase activity, metal ion  binding

Subcellular Location

Cytosol

Expression

Expressed in several tissues, including brain and heart.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic nucleotide phosphodiesterase 1C

Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C 

Cam PDE 1C 

HCAM3 

HSPDE1C1A 

Human 3'5' cyclic nucleotide Phosphodiesterase

PDE 1C 

Phosphodiesterase 1C 

Phosphodiesterase 1C calmodulin dependent

General Information

Product

PDE1C Antibody

Description

Affinity Purified Pan Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A) Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on PDE1C gene. The sequence was taken within amino acid region 10-70.

Accession #

GenBank: U40371.1 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

This antibody is labeled as C-epitope, because it was taken closer to the C-terminus in relation to our other PDE1C antibody (PD1C-301AP).

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:40-1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:40-1:100

Immunofluorescence

1:200

Immunohistochemistry

1:40-1:100

Immunoprecipitation

1:200

Western Blot

1:500-1:2,000

Protein

Uniprot #

Q14123

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.

Molecular Function

Hydrolase, calmodulin-dependent cyclic-nucleotide phosphodiesterase activity, metal ion  binding

Subcellular Location

Cytosol

Expression

Expressed in several tissues, including brain and heart.

Structure

Homodimer

Alternative Nomenclature

5''-cyclic nucleotide phosphodiesterase 1C antibody

Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C antibody

Cam PDE 1C antibody

HCAM3 antibody

HSPDE1C1A antibody

Human 3'5' cyclic nucleotide Phosphodiesterase antibody

PDE 1C antibody

Phosphodiesterase 1C antibody

Phosphodiesterase 1C calmodulin dependent antibody

General Information

Product

PDE4 Antibody

Description

Phosphodiesterase 4 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide common to most PDE4 subtypes: PDE4A, PDE4D, PDE4B

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Overview

Phosphodiesterase specific for extracellular cAMP. Involved in the degradation of extracellular cAMP specifically during multicellular development.

Molecular Function

3',5'-cyclic-nucleotide phosphodiesterase activity; cAMP catabolic process; signal transduction; Hydrolase

Subcellular Location

Cell membrane; Multipass membrane protein

Expression

Low expression during growth and cell aggregation (up to 6 hours), maximally increases after 9 hours of starvation, during the mound stage. Remains expressed at significant levels during the other stages of multicellular development.

Structure

Belongs to the cyclic nucleotide phosphodiesterase family.

Alternative Nomenclature

PDE4A antibody

PDE4B antibody

PDE4C antibody

PDE4D antibody

Phosphodiesterase 4 antibody

Phosphodiesterase 4A antibody

Phosphodiesterase 4B antibody

Phosphodiesterase 4C antibody

Phosphodiesterase 4D antibody