General Information

Product

PDE5A Antibody

Description

Affinity Purified cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC-P, IHC-Fr, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig, Rat

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:1,000

Protein

Uniprot #

O76074

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific 3'5' cyclic phosphodiesterase antibody

cGMP specific phosphodiesterase PDE5A2 antibody

cGMP specific phosphodiesterase type 5A antibody

cGMP-specific 3'' antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A1 antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

Phospho-PDE3A Antibody

Description

Affinity Purified Phosphorylated cGMP-inhibited 3',5'-cyclic phosphodiesterase A Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 200-300 on mouse PDE3A protein. Phosphorylated Serine 290 & 292 residues.

Accession #

NCBI:  NP_061249.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.0-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Z0X4

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Membrane; Multipass membrane protein

Alternative Nomenclature

5''-cyclic phosphodiesterase A antibody

cAMP phosphodiesterase myocardial cGMP inhibited antibody

CGI PDE antibody

CGI PDE A antibody

cGMP inhibited 3' 5' cyclic phosphodiesterase A antibody

Cyclic GMP-inhibited phosphodiesterase A antibody

Human cGMP inhibited cAMP phosphodiesterase mRNA antibody

PDE 3A antibody

Phosphodiesterase 3A antibody

Phosphodiesterase 3A cGMP inhibited antibody

General Information

Product

Phospho-PDE5A Antibody

Description

Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Pig, Rat, Sheep

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted 

Accession #

GenBank: EAX05277.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.0-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0000EE2B22

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific phosphodiesterase type 5A antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A1 antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

PDE2A Antibody

Description

Affinity Purified Phosphodiesterase 2A, cGMP-stimulated Antibody C-epitope

Verified Applications

CM, ELISA, FRET, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique C-terminal amino acid sequence on PDE2A gene.

Accession #

GenBank: EAW74861.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250-1:1,000

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250-1:1,000

Immunofluorescence

1:150-1:1,000

Immunohistochemistry

1:250-1:1,000

Immunoprecipitation

1:200

Western Blot

1:250-1:1,000

Protein

Uniprot #

UPI000003B340

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Molecular Function

Hydrolase, cAMP, cGMP, Metal-binding, Angiogenesis

Subcellular Location

Multi-pass membrane protein

Expression

bundant in adipose tissues.

Structure

Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

cGMP dependent 3'5' cyclic phosphodiesterase antibody

cGMP stimulated phosphodiesterase 1 antibody

cGMP stimulated phosphodiesterase 4 antibody

cGMP-dependent 3'' antibody

CGS PDE antibody

Cyclic GMP stimulated phosphodiesterase antibody

PDE 2A antibody

PDE 2A1 antibody

PDE 2A4 antibody

PDE2A antibody

Phosphodiesterase 2A cGMP stimulated antibody

General Information

Product

PDE2A Antibody

Description

Affinity Purified Phosphodiesterase 2A, cGMP-stimulated Antibody N-epitope

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on PDE2A gene.

Accession #

GenBank: EAW74861.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250-1:1,000

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250-1:1,000

Immunofluorescence

1:150-1:1,000

Immunohistochemistry

1:250-1:1,000

Immunoprecipitation

1:200

Western Blot

1:250-1:1,000

Protein

Uniprot #

UPI000003B340

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Molecular Function

Hydrolase, cAMP, cGMP, Metal-binding, Angiogenesis

Subcellular Location

Multi-pass membrane protein

Expression

bundant in adipose tissues.

Structure

Interacts with PIK3CG (By similarity). Interacts with RAPGEF3 and PIK3R6.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

cGMP dependent 3'5' cyclic phosphodiesterase antibody

cGMP stimulated phosphodiesterase 1 antibody

cGMP stimulated phosphodiesterase 4 antibody

cGMP-dependent 3'' antibody

CGS PDE antibody

Cyclic GMP stimulated phosphodiesterase antibody

PDE 2A antibody

PDE 2A1 antibody

PDE 2A4 antibody

PDE2A antibody

Phosphodiesterase 2A cGMP stimulated antibody

General Information

Product

PDE4D2 Antibody Affinity Purified

Description

Affinity Purified Phosphodiesterase 4D2 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on PDE4D2 variant gene.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.76 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Apical cell membrane, Cytoplasm, Membrane

Expression

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Structure

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1.

Alternative Nomenclature

cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

DKFZp686M11213 antibody

cAMP-specific 3'' antibody

cyclic AMP phosphodiesterase antibody

DPDE 3 antibody

FLJ97311 antibody

HSPDE4D antibody

PDE43 antibody

PDE4DN2 antibody

Phosphodiesterase 4D, cAMP specific (dunce) antibody

Phosphodiesterase E3 dunce homolog Drosophila antibody

STRK 1 antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from near the N terminus common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:2,000

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase 8A antibody

Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody Mid-Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from mid-region common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:2,000

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase 8A antibody

Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase antibody

General Information

Product

PDE8A Antibody

Description

Affinity Purified Pan Phosphodiesterase 8A Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic cyclic peptide from C terminus common to all PDE8A variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.

Molecular Function

3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding

Subcellular Location

Cytosol

Expression

Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.

Structure

Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily.  Contains 1 PAC (Pas-associated C-terminal) domain.  Contains 1 PAS (PER-ARNT-SIM) domain.

Alternative Nomenclature

5''-cyclic phosphodiesterase 8A antibody

cAMP specific cyclic nucleotide phosphodiesterase 8A antibody

FLJ16150 antibody

High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody

HsT19550 antibody

PDE 8A antibody

Phosphodiesterase8A antibody

Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody

General Information

Product

PDE3B Antibody

Description

Affinity Purified cGMP-inhibited 3',5'-cyclic phosphodiesterase B Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1060-1110 on human PDE3B protein.

Accession #

GenBank:  BAA09306

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13370

Overview

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Regulates cAMP binding of RAPGEF3.

Molecular Function

Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Membrane; Multipass membrane protein

Expression

Abundant in adipose tissues.

Structure

Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6.

Alternative Nomenclature

5''-cyclic phosphodiesterase B antibody

adipocyte cAMP phosphodiesterase cGMP inhibited antibody

AI847709 antibody

cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody

CGIP1 antibody

CGIPDE B antibody

cGMP inhibited 3 5 cyclic phosphodiesterase B antibody

CGMP inhibited cAMP phophodiesterase antibody

cGMP-inhibited 3'' antibody

Cyclic GMP-inhibited phosphodiesterase B antibody

Cyclic nucleotide phosphodiesterase antibody

HcGIP1 antibody

Phosphodiesterase 3B cGMP inhibited antibody